Wentz-Hunter K, Potashkin J
Department of Pharmacology and Molecular Biology, Finch University of Health Sciences/The Chicago Medical School, North Chicago, IL 60064, USA.
Nucleic Acids Res. 1996 May 15;24(10):1849-54. doi: 10.1093/nar/24.10.1849.
The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59 using the yeast two-hybrid system and have identified a homolog of the hsU2AF35 subunit. The S. pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59 that interacts with spU2AF23 is similar to the region in which the human small and large subunits interact.
人类剪接因子U2辅助因子(hsU2AF)由两个相互作用的亚基组成,分子量分别为65 kDa和35 kDa。此前我们鉴定出了人类大亚基的粟酒裂殖酵母同源物spU2AF59。我们利用酵母双杂交系统筛选了裂殖酵母cDNA文库,以寻找与spU2AF59相互作用的蛋白质,并鉴定出了hsU2AF35亚基的同源物。粟酒裂殖酵母U2AF小亚基是一个单拷贝基因,编码一种与人类小亚基氨基酸序列一致性为55%、相似性为17%的蛋白质。与人类蛋白质不同,酵母蛋白质缺乏富含精氨酸/丝氨酸的区域。spU2AF小亚基的预测分子量为23 kDa。spU2AF59与spU2AF23相互作用的区域,类似于人类小亚基和大亚基相互作用的区域。