Mizushina Y, Tanaka N, Yagi H, Kurosawa T, Onoue M, Seto H, Horie T, Aoyagi N, Yamaoka M, Matsukage A, Yoshida S, Sakaguchi K
Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Chiba-ken, Japan.
Biochim Biophys Acta. 1996 Sep 11;1308(3):256-62. doi: 10.1016/0167-4781(96)00121-2.
The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.
研究了真核生物DNA聚合酶与脂肪酸之间的体外关系。一些脂肪酸在体外强烈抑制DNA聚合酶α和/或β的活性。亚油酸抑制作用的动力学表明，就DNA模板和底物（dTTP）而言，DNA聚合酶α受到非竞争性抑制，而DNA聚合酶β受到DNA和底物两者的竞争性抑制。
