Liu Z, Hultin L E, Cumberland W G, Hultin P, Schmid I, Matud J L, Detels R, Giorgi J V
Department of Epidemiology, UCLA School of Public Health, USA.
Cytometry. 1996 Mar 15;26(1):1-7. doi: 10.1002/(SICI)1097-0320(19960315)26:1<1::AID-CYTO1>3.0.CO;2-L.
Relative fluorescence intensity measurements from a flow cytometer were used to evaluate expression of CD38 and HLA-DR antigens. These molecules are associated with cellular activation and are present at increased levels on the CD8+ lymphocytes of HIV-infected subjects. In the current study, the prognostic value of mean fluorescence intensity measurements of CD38 and HLA-DR on CD8+ cells was compared to results from our previous study in which we reported prognostic value for an elevated percentage of CD8+ cells that were positive for expression of the CD38 antigen (Giorgi et al.: JAIDS 6:904-912, 1993). Using the proportional hazards model, elevated mean fluorescence intensity of CD38 expression on CD8+ cells had prognostic value for development of AIDS that was almost identical to the prognostic value of the percentage of CD8+ cells that were positive for expression of CD38. This prognostic value was in addition to that provided by the patient's CD4+ cell measurement. To our knowledge, this is the first report that a measurement of fluorescence intensity can be used as a prognostic marker in an immunodeficiency disease. Efforts are needed to establish methods that will allow widespread application of this observation in the clinical management of HIV-infected subjects.
利用流式细胞仪进行相对荧光强度测量，以评估CD38和HLA - DR抗原的表达。这些分子与细胞活化相关，且在HIV感染受试者的CD8 +淋巴细胞上水平升高。在本研究中，将CD8 +细胞上CD38和HLA - DR平均荧光强度测量的预后价值与我们之前的研究结果进行了比较，在之前的研究中，我们报告了CD38抗原表达阳性的CD8 +细胞百分比升高的预后价值（Giorgi等人：《获得性免疫缺陷综合征杂志》6:904 - 912，1993年）。使用比例风险模型，CD8 +细胞上CD38表达的平均荧光强度升高对艾滋病发展具有预后价值，这与CD38表达阳性的CD8 +细胞百分比的预后价值几乎相同。这种预后价值是在患者CD4 +细胞测量所提供的价值之外的。据我们所知，这是第一份关于荧光强度测量可作为免疫缺陷疾病预后标志物的报告。需要努力建立能够使这一观察结果在HIV感染受试者的临床管理中广泛应用的方法。
