酿酒酵母第十五号染色体右臂上一个35.6千碱基区域的分析。
Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV.
作者信息
Bordonné R, Camasses A, Madania A, Poch O, Tarassov I, Winsor B, Martin R
机构信息
UPR 9005 MMDCD du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
出版信息
Yeast. 1997 Jan;13(1):73-83. doi: 10.1002/(SICI)1097-0061(199701)13:1<73::AID-YEA52>3.0.CO;2-M.
We report the sequence of a 35,600 bp fragment covering the PET123 region on the right arm of chromosome XV from Saccharomyces cerevisiae. This region contains 19 possible open reading frames (ORFs) of which 16 are non-overlapping ORFs. Eight ORFs correspond to the SPP2, SMP3, PDR5, NFI1, PUP1, PET123 and MTR10 loci, described previously. Two ORFs correspond to yeast homologues of genes from other organisms: O3530 is a member of the large ribosomal subunit protein L13 family and O3560 (SME1 gene) is a 94-codon ORF and is a homologue of the mammalian SmE spliceosomal core protein. Three ORFs (O3513, O3521, O3548) present significant similarities to proteins of unknown function and three ORFs (O3510, O3536, O3545) lack homology to sequences within the databases screened.
我们报道了来自酿酒酵母第十五号染色体右臂上覆盖PET123区域的一段35,600 bp片段的序列。该区域包含19个可能的开放阅读框（ORF），其中16个是不重叠的ORF。八个ORF分别对应于之前描述的SPP2、SMP3、PDR5、NFI1、PUP1、PET123和MTR10基因座。两个ORF对应于其他生物体基因的酵母同源物：O3530是大核糖体亚基蛋白L13家族的成员，O3560（SME1基因）是一个94个密码子的ORF，是哺乳动物SmE剪接体核心蛋白的同源物。三个ORF（O3513、O3521、O3548）与功能未知的蛋白质具有显著相似性，三个ORF（O3510、O3536、O3545）与所筛选数据库中的序列缺乏同源性。
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