McGarvey M J, Goldin R D, Karayiannis P, Thomas H C
Department of Medicine, Imperial College School of Medicine at St Mary's, London, UK.
J Viral Hepat. 1996 Mar;3(2):67-73. doi: 10.1111/j.1365-2893.1996.tb00083.x.
A recombinant protein corresponding to part of the amino-terminal domain of hepatitis B virus (HBV) polymerase was expressed in Escherichia coli. Antisera raised against this protein stained hepatocytes, from human liver biopsies, predominantly in the nucleus but some cytoplasmic staining was also observed. No staining was observed in hepatocytes from uninfected patients. Liver biopsies, taken from patients who were infected with human immunodeficiency virus (HIV) as well as HBV showed more intense staining with these antisera than that seen in patients who were infected with HBV alone. The staining pattern suggests that either the whole HBV polymerase protein, or a portion encoding the amino-terminal domain, is translocated to the nucleus. This event may be an important early step in replication of the HBV genome.
一种与乙型肝炎病毒（HBV）聚合酶氨基末端结构域部分相对应的重组蛋白在大肠杆菌中表达。用针对该蛋白产生的抗血清对人肝活检组织中的肝细胞进行染色，主要在细胞核中，但也观察到一些细胞质染色。未感染患者的肝细胞中未观察到染色。从感染了人类免疫缺陷病毒（HIV）以及HBV的患者身上获取的肝活检组织，与仅感染HBV的患者相比，用这些抗血清染色时显示出更强的染色。染色模式表明，要么是整个HBV聚合酶蛋白，要么是编码氨基末端结构域的部分，被转运到细胞核中。这一事件可能是HBV基因组复制的一个重要早期步骤。
