Lund A, Knudsen S M, Vissing H, Clark B, Tommerup N
Department of Biostructural Chemistry, Aarhus University, Aarhus, Denmark.
Genomics. 1996 Sep 1;36(2):359-61. doi: 10.1006/geno.1996.0475.
The human elongation factor 1alpha gene family consists of at least 2 actively transcribed genes, EEF1A and EEF1A2, and more than 18 homologous loci. EEF1A2 is expressed in a tissue-specific manner, whereas EEF1A is expressed ubiquitously, and both of them can function in translation. An EEF1A cDNA probe has previously been shown to cross-hybridize with several human chromosomes, but the location of the functional gene has not been established. We have mapped the functional EEF1A gene to 6q14 by combined fluorescence in situ hybridization (FISH) and PCR analysis of a somatic cell hybrid panel and mapped EEF1A2 to 20q13.3 by FISH. In addition, the 11 strongest cross-hybridizing loci (EEF1AL2-EEF1AL13) were mapped by FISH to 12p12, 9q34, 7p15-p21, 19q13, 3q26-q27, 7q33-q35, 1p13-p22, 2q12-q14, 5p12-q11, 1q31-q32, and Xq21.
人类延伸因子1α基因家族由至少2个活跃转录的基因EEF1A和EEF1A2以及18个以上的同源基因座组成。EEF1A2以组织特异性方式表达，而EEF1A在全身广泛表达，二者均在翻译过程中发挥作用。先前已证明EEF1A cDNA探针可与几条人类染色体发生交叉杂交，但功能性基因的位置尚未确定。我们通过荧光原位杂交（FISH）和体细胞杂交板的PCR分析，将功能性EEF1A基因定位于6q14，并通过FISH将EEF1A2定位于20q13.3。此外，通过FISH将11个最强交叉杂交基因座（EEF1AL2 - EEF1AL13）定位于12p12、9q34、7p15 - p21、19q13、3q26 - q27、7q33 - q35、1p13 - p22、2q12 - q14、5p12 - q11、1q31 - q32和Xq21。
