Gislason H, Røkke O, Svanes K
Surgical Research Laboratory, University of Bergen, Norway.
Eur Surg Res. 1996 Jul-Aug;28(4):278-86. doi: 10.1159/000129467.
This study was performed to examine if the cytokines interleukin-1 (IL-1), interleukin-6 (IL-6) or tumor necrosis factor (TNF) are released from the gastric mucosa during acute mucosal damage, and if the generation of these cytokines is affected by indomethacin. Cat stomachs were exposed to 2 M NaCl for 10 min followed by luminal perfusion at pH 1. Gastric mucosal blood flow was determined by radioactive microspheres, portal vein blood flow by transit-time flowmetry, and H+ back diffusion/secretion by pH-stat titration. Concentrations of active cytokines and of histamine in aortic and portal vein blood were measured by bioassay and RIA, respectively. Active IL-6, but not IL-1 and TNF, is released from the gastric mucosa during acute mucosal damage by 2 M NaCl and acid back diffusion. Indomethacin increased mucosal injury and enhanced the TNF generation but reduced the release of IL-6 from the gastric mucosa. We conclude that IL-1 and TNF probably do not play an important modulating role during acute gastric mucosal damage. The generation of IL-6 may, however, contribute to mucosal protection.
本研究旨在探讨在急性黏膜损伤期间，细胞因子白细胞介素-1（IL-1）、白细胞介素-6（IL-6）或肿瘤坏死因子（TNF）是否从胃黏膜释放，以及这些细胞因子的生成是否受吲哚美辛影响。将猫胃暴露于2 M NaCl中10分钟，随后在pH 1条件下进行腔内灌注。通过放射性微球测定胃黏膜血流量，通过渡越时间血流仪测定门静脉血流量，通过pH计滴定法测定H⁺反向扩散/分泌。分别通过生物测定法和放射免疫分析法（RIA）测量主动脉和门静脉血中活性细胞因子和组胺的浓度。在急性黏膜损伤期间，2 M NaCl和酸反向扩散可使胃黏膜释放活性IL-6，但不释放IL-1和TNF。吲哚美辛增加了黏膜损伤并增强了TNF的生成，但减少了胃黏膜中IL-6的释放。我们得出结论，IL-1和TNF在急性胃黏膜损伤期间可能不发挥重要的调节作用。然而，IL-6的生成可能有助于黏膜保护。
