Rodríguez J L, Orós J, Rodríguez F, Poveda J B, Ramírez A, Fernández A
Department of Histology and Pathological Anatomy, Veterinary Faculty, University of Las Palmas of Gran Canaria, Spain.
J Comp Pathol. 1996 May;114(4):373-84. doi: 10.1016/s0021-9975(96)80013-2.
In a population of 700 goats, 150 died; of these, 29 were necropsied. Ten of the 29 goats had pleuropneumonia. Mycoplasma mycoides subsp. mycoides (Large Colony) (MmmLC), Mycoplasma mycoides subsp. capri (Mmc), and Pasteurella multocida were isolated from five of the pleuropneumonic goats. Gross and microscopical lesions were typical of caprine pleuropneumonia (CPP), with bronchopneumonia, fibrinopurulent or fibrinonecrotic pleuropneumonia and dilatation of the interlobular septa and pleura. Immunohistochemical examination with antisera against MmmLC and Mmc showed mycoplasma antigens in all 10 goats with CPP. In all cases, both MmmLC and Mmc antigens were detected together. Mycoplasma antigens were present in the lumina of the airways and alveoli, mainly inside the cytoplasm of neutrophils and macrophages, but extracellular antigen was demonstrated in areas of necrosis. Pasteurella antigens were detected in four of the 10 animals with CPP. From the histological, immunohistochemical and microbiological results it was concluded that the two mycoplasmas, acting together, caused the pleuropneumonia, with P. multocida playing a subsidiary synergistic role.
在700只山羊群体中，150只死亡；其中29只进行了尸检。29只山羊中有10只患有胸膜肺炎。从5只患胸膜肺炎的山羊中分离出了丝状支原体丝状亚种（大菌落）（MmmLC）、丝状支原体山羊亚种（Mmc）和多杀性巴氏杆菌。大体和显微镜下病变为典型的山羊胸膜肺炎（CPP），伴有支气管肺炎、纤维脓性或纤维坏死性胸膜肺炎以及小叶间隔和胸膜扩张。用抗MmmLC和Mmc抗血清进行免疫组织化学检查显示，所有10只患CPP的山羊中均有支原体抗原。在所有病例中，MmmLC和Mmc抗原均同时被检测到。支原体抗原存在于气道和肺泡腔中，主要在中性粒细胞和巨噬细胞的细胞质内，但在坏死区域可见细胞外抗原。在10只患CPP的动物中有4只检测到巴氏杆菌抗原。从组织学、免疫组织化学和微生物学结果得出结论，两种支原体共同作用导致了胸膜肺炎，多杀性巴氏杆菌起辅助协同作用。
