Rodríguez F, Martín de las Mulas J, Herráez P, Sánchez Vizcaíno J M, Fernández A
Departamento de Anatomía Patológica, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Spain.
J Comp Pathol. 1996 May;114(4):399-406. doi: 10.1016/s0021-9975(96)80015-6.
A study was made of the action of African swine fever virus (ASFV) on the bone marrow of 12 miniature pigs inoculated intramuscularly with the moderately virulent ASFV isolate E75 and killed 2 to 12 days after infection. A sequential description is provided of the histological lesions of the bone marrow in the experimental animals, which developed haemorrhagic lesions from 6 days after inoculation onwards. Immunohistochemical techniques were used to demonstrate the viral protein VP73 and immunoglobulins (IgG and IgM) in formalin-fixed and paraffin wax-embedded samples of bone marrow tissue. The immunohistological results, platelet counts, viraemia, and anti-ASFV immunoglobulin titres all indicated that thrombocytopoiesis impairment by direct viral action plays a role in the progressive thrombocytopenia characteristic of infection by moderately virulent ASFV isolates.
对12只经肌肉注射中等毒力非洲猪瘟病毒（ASFV）分离株E75接种并在感染后2至12天处死的小型猪的骨髓进行了研究。对实验动物骨髓的组织学病变进行了连续描述，这些病变从接种后6天开始出现出血性病变。采用免疫组织化学技术在福尔马林固定、石蜡包埋的骨髓组织样本中检测病毒蛋白VP73和免疫球蛋白（IgG和IgM）。免疫组织学结果、血小板计数、病毒血症和抗ASFV免疫球蛋白滴度均表明，直接病毒作用导致的血小板生成受损在中等毒力ASFV分离株感染所特有的进行性血小板减少中起作用。
