Bautista M J, Gómez-Villamandos J C, Carrasco L, Ruíz-Villamor E, Salguero F J, Sierra M A
Department of Pathology, Faculty of Veterinary, University of Córdoba, Spain.
Histol Histopathol. 1998 Jul;13(3):713-20. doi: 10.14670/HH-13.713.
Interpretation of changes in bone marrow during infectious processes is quite complex. This paper reports bone marrow lesions observed in pigs inoculated with a moderately virulent ASF virus strain and studies their relationship to the pathogenesis of the disease. In this work, we have carried out the structural and ultrastructural study of the bone marrow of 14 Large White x Landrace pigs that were inoculated by the intramuscular route with 10(5) 50% hemodsorbing doses (HAD50) of the Dominican Republic'78 ASF virus strain. The inoculated pigs were killed at 3, 5, 7, 9, 11, 13, 15 and 17 days postinjection. Analysis of cells and structures belonging to the two main bone-marrow compartments, the hematopoietic cells and the hematopoietic micro-environment, showed that after inoculation with a moderately virulent strain, the most significant changes occurred in macrophages and megakaryocytes, consisting in virus replication in these cell populations and apoptosis of megakaryocytes, related with the sudden and transitory thrombocytopenia detected in the subacute ASF.
感染过程中骨髓变化的解读相当复杂。本文报告了接种中等毒力非洲猪瘟病毒株的猪所观察到的骨髓病变，并研究了它们与疾病发病机制的关系。在这项工作中，我们对14头大白猪与长白猪的杂交猪进行了骨髓的结构和超微结构研究，这些猪通过肌肉注射接种了10(5) 50%血液吸附剂量（HAD50）的多米尼加共和国78株非洲猪瘟病毒株。在注射后第3、5、7、9、11、13、15和17天处死接种的猪。对属于骨髓两个主要成分，即造血细胞和造血微环境的细胞和结构进行分析表明，接种中等毒力毒株后，巨噬细胞和巨核细胞发生了最显著的变化，包括这些细胞群体中的病毒复制和巨核细胞凋亡，这与亚急性非洲猪瘟中检测到的突然且短暂的血小板减少有关。
