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本文引用的文献

1
Comparison of culture methods for isolation of nontuberculous mycobacteria from surface waters.比较从地表水中分离非结核分枝杆菌的培养方法。
Appl Environ Microbiol. 2010 Jun;76(11):3514-20. doi: 10.1128/AEM.02659-09. Epub 2010 Apr 2.
2
Determination of genotypic diversity of Mycobacterium avium subspecies from human and animal origins by mycobacterial interspersed repetitive-unit-variable-number tandem-repeat and IS1311 restriction fragment length polymorphism typing methods.应用分枝杆菌间隔重复单元可变数目串联重复和 IS1311 限制片段长度多态性分型方法检测人源和动物源鸟分枝杆菌复合群的基因型多样性。
J Clin Microbiol. 2010 Apr;48(4):1026-34. doi: 10.1128/JCM.01869-09. Epub 2010 Jan 27.
3
Aerosolization of mycobacteria and legionellae during dental treatment: low exposure despite dental unit contamination.牙科治疗过程中分枝杆菌和军团菌的雾化：尽管牙科设备受到污染，但暴露风险较低。
Environ Microbiol. 2007 Nov;9(11):2836-43. doi: 10.1111/j.1462-2920.2007.01395.x.
4
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.MEGA4：分子进化遗传学分析（MEGA）软件版本4.0。
Mol Biol Evol. 2007 Aug;24(8):1596-9. doi: 10.1093/molbev/msm092. Epub 2007 May 7.
5
Comparison of three methods for rapid identification of mycobacterial clinical isolates to the species level.三种将分枝杆菌临床分离株快速鉴定到种水平方法的比较。
J Clin Microbiol. 2007 Jun;45(6):1898-903. doi: 10.1128/JCM.02253-06. Epub 2007 Mar 14.
6
Rapid detection and species identification of Mycobacterium spp. using real-time PCR and DNA-microarray.使用实时聚合酶链反应和DNA微阵列技术快速检测和鉴定分枝杆菌属菌种。
J Microbiol Methods. 2006 Jul;66(1):116-24. doi: 10.1016/j.mimet.2005.10.016. Epub 2005 Dec 19.
7
Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens.一种用于检测临床标本中曲霉菌种DNA的巢式定性实时PCR检测方法的开发。
J Clin Microbiol. 2005 Oct;43(10):5366-8. doi: 10.1128/JCM.43.10.5366-5368.2005.
8
Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65).通过分析热休克蛋白65基因（hsp65）对分枝杆菌菌种进行鉴别。
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1649-1656. doi: 10.1099/ijs.0.63553-0.
9
A quantitative PCR assay for the assessment of DNA degradation in forensic samples.一种用于评估法医样本中DNA降解情况的定量PCR检测方法。
Forensic Sci Int. 2006 Apr 20;158(1):14-26. doi: 10.1016/j.forsciint.2005.04.034. Epub 2005 Jun 3.
10
Comparative evaluation of polymerase chain reaction and restriction enzyme analysis: two amplified targets, hsp65 and rpoB, for identification of cultured mycobacteria.聚合酶链反应与限制性酶切分析的比较评估：用于鉴定培养分枝杆菌的两个扩增靶点，hsp65和rpoB。
Diagn Microbiol Infect Dis. 2005 Mar;51(3):165-71. doi: 10.1016/j.diagmicrobio.2004.09.006.

建立一种实时 qPCR 方法，用于检测和计数地表水中的分枝杆菌属。

Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.

机构信息

Université Paris-Est, Laboratoire Eau Environnement Systèmes Urbains UMR MA 102-AgroParisTech, 6-8 avenue Blaise Pascal Cité Descartes, FR 77455 Champs sur Marne, France.

出版信息

Appl Environ Microbiol. 2010 Nov;76(21):7348-51. doi: 10.1128/AEM.00942-10. Epub 2010 Sep 17.

DOI:10.1128/AEM.00942-10
PMID:20851986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2976254/
Abstract

A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).

摘要

建立了一种实时荧光定量 PCR 方法，用于从环境样本中检测和计数分枝杆菌属。并与另外两种已描述的方法进行了比较。结果表明，我们的方法（针对 16S rRNA）比以前报道的两种针对另一个 16S rRNA 基因座和 hsp65 基因的实时荧光定量 PCR 方法更具特异性（分别为 100%、44%和 91%）。