建立一种实时 qPCR 方法，用于检测和计数地表水中的分枝杆菌属。
Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.
机构信息
Université Paris-Est, Laboratoire Eau Environnement Systèmes Urbains UMR MA 102-AgroParisTech, 6-8 avenue Blaise Pascal Cité Descartes, FR 77455 Champs sur Marne, France.
出版信息
Appl Environ Microbiol. 2010 Nov;76(21):7348-51. doi: 10.1128/AEM.00942-10. Epub 2010 Sep 17.
Abstract
A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).
摘要
建立了一种实时荧光定量 PCR 方法，用于从环境样本中检测和计数分枝杆菌属。并与另外两种已描述的方法进行了比较。结果表明，我们的方法（针对 16S rRNA）比以前报道的两种针对另一个 16S rRNA 基因座和 hsp65 基因的实时荧光定量 PCR 方法更具特异性（分别为 100%、44%和 91%）。
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