上游结合因子通过多种蛋白质相互作用以不依赖DNA的方式稳定Rib 1，即含有TATA结合蛋白的非洲爪蟾RNA聚合酶I转录因子。
Upstream binding factor stabilizes Rib 1, the TATA-binding-protein-containing Xenopus laevis RNA polymerase I transcription factor, by multiple protein interactions in a DNA-independent manner.
作者信息
Bodeker M, Cairns C, McStay B
机构信息
Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Scotland.
出版信息
Mol Cell Biol. 1996 Oct;16(10):5572-8. doi: 10.1128/MCB.16.10.5572.
Abstract
Initiation of RNA polymerase I transcription in Xenopus laevis requires Rib 1 and upstream binding factor (UBF). UBF and Rib 1 combine to form a stable transcription complex on the Xenopus ribosomal gene promoter. Here we show that Rib 1 comprises TATA-binding protein (TBP) and TBP-associated factor components. Thus, Rib 1 is the Xenopus equivalent of mammalian SL 1. In contrast to SL 1, Rib 1 is an unstable complex that readily dissociates into TBP and associated components. We identify a novel function for UBF in stabilizing Rib 1 by multiple protein interactions. This stabilization occurs in solution in a DNA-independent manner. These results may partially explain the difference in UBF requirement between Xenopus and mammalian systems.
摘要
非洲爪蟾中RNA聚合酶I转录的起始需要Rib 1和上游结合因子（UBF）。UBF和Rib 1结合在非洲爪蟾核糖体基因启动子上形成稳定的转录复合物。在此我们表明，Rib 1包含TATA结合蛋白（TBP）和TBP相关因子成分。因此，Rib 1相当于哺乳动物的SL 1。与SL 1不同，Rib 1是一种不稳定的复合物，很容易解离成TBP和相关成分。我们通过多种蛋白质相互作用确定了UBF在稳定Rib 1方面的新功能。这种稳定作用在溶液中以不依赖DNA的方式发生。这些结果可能部分解释了非洲爪蟾和哺乳动物系统在UBF需求上的差异。
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本文引用的文献
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The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor.物种特异性的RNA聚合酶I转录因子SL-1与上游结合因子结合。
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