氯霉素对变形虫属一种菌株中细菌内共生体的影响。

Effect of chloramphenicol on bacterial endosymbiotes in a strain of Amoeba proteus.

作者信息

Jeon K W, Hah J C

出版信息

J Protozool. 1977 May;24(2):289-93. doi: 10.1111/j.1550-7408.1977.tb00979.x.

DOI:10.1111/j.1550-7408.1977.tb00979.x

Abstract

The effect of chloramphenicol (CAP) on the bacterial endosymbiotes of a strain of Amoeba proteus was studied by growing the symbiotic amebae in media containing 0.5-1.6 mg/ml CAP for up to 4 weeks. Treatments with CAP caused such ultrastructural changes as expansion of the nuclear zone and deformation of symbiotes. The CAP treatment also damaged the mitochondria, e.g. disappearance of central and protrusion of peripheral cristae. Number of bacteria per ameba decreased to less than 10% of control in CAP-containing media, but no viable amebae became completely free of symbiotes. The results supported previous studies that amebae were dependent on endosymbiotes.

摘要

通过在含有0.5 - 1.6毫克/毫升氯霉素（CAP）的培养基中培养共生变形虫长达4周，研究了氯霉素（CAP）对一株变形虫细菌内共生体的影响。用CAP处理导致了超微结构的变化，如核区扩大和共生体变形。CAP处理还损害了线粒体，例如中央嵴消失和外周嵴突出。在含CAP的培养基中，每个变形虫的细菌数量减少到对照的不到10%，但没有存活的变形虫完全没有共生体。这些结果支持了先前关于变形虫依赖内共生体的研究。