Cuajungco M P, Lees G J
Department of Psychiatry and Behavioural Science, University of Auckland School of Medicine, New Zealand.
Neuroreport. 1996 May 17;7(7):1301-4. doi: 10.1097/00001756-199605170-00017.
Intrahippocampal injections of zinc chloride (10 nmol) produce lesions in the rat hippocampus. A heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylene-diamine (TPEN), co-injected with zinc chloride in vivo, totally prevented zinc-induced neuronal loss. Furthermore, intrahippocampal injections of TPEN (10 nmol) were non-toxic when compared with controls. TPEN injections in vivo quenched the in vitro staining of brain sections for zinc (Timm's stain), particularly the staining of the hippocampal mossy fibres. This demonstrates that TPEN is taken up by zinc-containing neurones in vivo. The use of TPEN to block the actions of zinc could help define the possible role of zinc in the pathology of various neurological disorders.
海马体内注射氯化锌（10纳摩尔）会导致大鼠海马体出现损伤。一种重金属螯合剂N,N,N',N'-四（2-吡啶甲基）乙二胺（TPEN），在体内与氯化锌共同注射时，能完全防止锌诱导的神经元损失。此外，与对照组相比，海马体内注射TPEN（10纳摩尔）无毒。体内注射TPEN可消除脑切片中锌的体外染色（廷氏染色），尤其是海马苔藓纤维的染色。这表明TPEN在体内被含锌神经元摄取。使用TPEN来阻断锌的作用有助于明确锌在各种神经疾病病理学中可能发挥的作用。
