锌调控曲霉属真菌免疫显性抗原的生物合成
Zinc-regulated biosynthesis of immunodominant antigens from Aspergillus spp.
作者信息
Segurado M, López-Aragón R, Calera J A, Fernández-Abalos J M, Leal F
机构信息
Departamento de Microbiología y Genética, Universidad de Salamanca, 37007 Salamanca, Spain.
出版信息
Infect Immun. 1999 May;67(5):2377-82. doi: 10.1128/IAI.67.5.2377-2382.1999.
Abstract
ASPND1 and ASPF2 are immunodominant antigens from Aspergillus nidulans and A. fumigatus, respectively, that are readily synthesized in infections in the human host, as demonstrated by their reactivity with more than 80% of sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis. We demonstrate here that both antigens are exclusively produced under situations of low bioavailability of free Zn2+. Addition of micromolar concentrations of Zn2+ to the culture medium strongly stimulated Aspergillus growth but totally inhibited ASPND1 or ASPF2 production. This effect was specific, since other divalent metals had no effect. Removal of endogenous Zn2+ by a chelator also stimulated ASPND1 production, and the effect was specifically reversed by Zn2+. These results suggest a possible role of these antigens in the survival of the fungus in the lungs.
摘要
ASPND1和ASPF2分别是来自构巢曲霉和烟曲霉的免疫显性抗原，在人类宿主感染过程中易于合成，这一点已通过它们与超过80%的曲菌球或变应性支气管肺曲霉病患者血清的反应性得到证明。我们在此证明，这两种抗原仅在游离Zn2+生物利用度低的情况下产生。向培养基中添加微摩尔浓度的Zn2+可强烈刺激曲霉生长，但完全抑制ASPND1或ASPF2的产生。这种效应具有特异性，因为其他二价金属没有影响。用螯合剂去除内源性Zn2+也会刺激ASPND1的产生，而Zn2+可特异性逆转这种效应。这些结果表明这些抗原在真菌在肺部存活中可能发挥作用。
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