Huang Q, Lan X, Tong T, Wu X, Chen M, Feng X, Liu R, Tang Y, Zhu Z
Center for Medical Laboratory Science, Fuzhou General Hospital, Fujian, People's Republic of China.
J Clin Microbiol. 1996 Aug;34(8):2011-3. doi: 10.1128/jcm.34.8.2011-2013.1996.
A dot-immunogold filtration assay (DIGFA) for the rapid detection of reaginic antibody in the serum of syphilitic patients was developed. The assay was simple, rapid, and reproducible. The test completion time was 2 min, and the assay required no equipment. The positive dot was very obvious, and the results could easily be determined with the naked eye. A total of 350 serum samples were examined by DIGFA, the rapid plasma reagin test, and the fluorescent treponemal antibody-absorption test. The levels of agreement between DIGFA and the rapid reagin test and between DIGFA and the fluorescent treponemal antibody-absorption test were 100 and 98%, respectively. The results of clinical application indicated that DIGFA could be used as a routine screening test for syphilis.
建立了一种用于快速检测梅毒患者血清中反应素抗体的斑点免疫金过滤试验（DIGFA）。该试验简单、快速且可重复。检测完成时间为2分钟，无需设备。阳性斑点非常明显，结果可用肉眼轻松判定。采用DIGFA、快速血浆反应素试验和荧光密螺旋体抗体吸收试验对350份血清样本进行了检测。DIGFA与快速反应素试验之间以及DIGFA与荧光密螺旋体抗体吸收试验之间的一致性水平分别为100%和98%。临床应用结果表明，DIGFA可作为梅毒的常规筛查试验。
