Manjabacas M C, Valero E, García-Moreno M, Garrido C, Varón R
Departamento de Química-Física, Escuela Universitaria Polítecnica deAlbacete, Universidad de Castilla-La Mancha, Spain.
Bull Math Biol. 1996 Jan;58(1):19-41. doi: 10.1007/BF02458280.
A global kinetic analysis of a model consisting of an autocatalytic zymogen-activation process, in which an irreversible inhibitor competes with the zymogen for the active site of the proteinase, and a monitoring coupled reaction, in which the enzyme acts upon one of its substrates, is presented. This analysis is based on the progress curves of any of the two products released in the monitoring reaction. The general solution is applied to an important particular case in which rapid equilibrium conditions prevail. Finally, we suggest a procedure to predict whether the inhibition or activation route dominates in the steady state of the system. These results generalize our previous analysis of simpler mechanisms.
本文给出了一个模型的全局动力学分析，该模型由一个自催化酶原激活过程（其中一种不可逆抑制剂与酶原竞争蛋白酶的活性位点）和一个监测偶联反应（其中酶作用于其一种底物）组成。该分析基于监测反应中释放的两种产物中任一种的进程曲线。将一般解应用于快速平衡条件占主导的一个重要特殊情况。最后，我们提出了一种程序来预测在系统稳态中抑制或激活途径是否占主导。这些结果推广了我们之前对更简单机制的分析。
