Li G D, Krull I S, Cohen S A
Department of Chemistry, Northeastern University, Boston, MA 02115, USA.
J Chromatogr A. 1996 Feb 16;724(1-2):147-57. doi: 10.1016/0021-9673(95)00941-8.
6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (6-AQC) is a reagent used to increase the detection-sensitivity of amino acids and peptides in high-performance liquid chromatography with fluorescence detection. In this paper, the electrochemical characteristics of the derivatives of 6-AQC are described. Electrochemical detection of 6-AQC amino acids and peptide derivatives following reverse-phase HPLC are also reported. The response linearity of the derivatives on an amperometric detector was studied in the range of 5 pmol (0.5 mu M) to 2500 pmol (250 mu M). Approximately 2.5 pmol of the amino acid derivatives could be detected. The quantitative results of amino acids in plasma and a bovine serum albumin hydrolysate agreed well with values reported in the literature.
6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯（6-AQC）是一种用于在高效液相色谱荧光检测中提高氨基酸和肽检测灵敏度的试剂。本文描述了6-AQC衍生物的电化学特性。还报道了反相高效液相色谱后对6-AQC氨基酸和肽衍生物的电化学检测。研究了衍生物在安培检测器上5皮摩尔（0.5微摩尔）至2500皮摩尔（250微摩尔）范围内的响应线性。大约可检测到2.5皮摩尔的氨基酸衍生物。血浆和牛血清白蛋白水解物中氨基酸的定量结果与文献报道的值吻合良好。
