通过姐妹染色单体交换测定几丁质和壳聚糖的抗遗传毒性活性。

Antigenotoxic activities of chitin and chitosan as assayed by sister chromatid exchange.

作者信息

Ohe T

机构信息

Department of Food and Nutrition Science, Kyoto Women's University, Japan.

出版信息

Sci Total Environ. 1996 Mar 1;181(1):1-5. doi: 10.1016/0048-9697(95)04949-5.

DOI:10.1016/0048-9697(95)04949-5

PMID:8819835

Abstract

The antigenotoxic activities of chitin and chitosan were studied using sister chromatid exchange assay by examining the adsorption of four kinds of mutagens. These two dietary animal fibers showed similar patterns in reducing the genotoxicity of aqueous solutions of the hydrophobic mutagens, 4-nitroquinoline-N-oxide and dinitropyrene, in distilled water. Under similar conditions, the antigenotoxic activities of chitin and chitosan for mitomycin C were 87 and 0%, and those for adriamycin were 47 and 78%, respectively. In addition, the antigenotoxic activity of both fibers for MMC was affected by the pH value of the aqueous solution between 2.5 and 7.2, but that of ADM was not. The results demonstrate that chitin and chitosan may have protective effects against environmental mutagens by adsorbing them in ionic and nonionic solutions.

摘要

通过检测四种诱变剂的吸附情况，利用姐妹染色单体交换试验研究了几丁质和壳聚糖的抗诱变活性。这两种膳食动物纤维在降低蒸馏水中疏水性诱变剂4-硝基喹啉-N-氧化物和二硝基芘水溶液的遗传毒性方面表现出相似的模式。在类似条件下，几丁质和壳聚糖对丝裂霉素C的抗诱变活性分别为87%和0%，对阿霉素的抗诱变活性分别为47%和78%。此外，两种纤维对丝裂霉素C的抗诱变活性受水溶液pH值（2.5至7.2）的影响，但对阿霉素的抗诱变活性不受影响。结果表明，几丁质和壳聚糖可能通过在离子和非离子溶液中吸附环境诱变剂而对其具有保护作用。