Suzuki K, Tsugawa R
Department of Urology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Scanning Microsc. 1995;9(4):1089-95; discussion 1095-6.
The aim of this study was to examine the effect of sodium pentosan polysulfate (SPP) in an undiluted urine system and to study its relative affinity to calcium oxalate (CaOx) crystals in the presence or absence of heparan sulfate (HS). CaOx crystals were induced with an overload of oxalate above the metastable limit in spun and filtered urine (SF) and ultrafiltered urine (UF). Then, the crystals were dissolved with EDTA (ethylenediaminetetraacetic acid), electrodialysed and lyophilized. The polyanions, HS or SPP were added to the UF prior to the addition of oxalate. Polyanions in crystal matrices were examined by cellulose acetate electrophoresis. Crystal volume and size were suppressed according to the increase of the concentration of SPP when compared with those of the UF. Scanning electron microscopy (SEM) showed marked aggregation of the crystals in the UF and no aggregation in the presence of SPP. HS was the only polyanion found in CaOx crystals formed after overload of oxalate in SF. Crystals formed in UF did not contain any polyanions. When SPP was added to UF, SPP appeared in the crystal matrix in accordance with its concentration. Once HS in physiological concentration was added to the UF containing SPP, HS and SPP obtained from crystals were strongly stained with Alcian blue in electrophoretic study, where SPP is stained stronger than HS. These results suggest that SPP strongly binds to CaOx crystals as well as HS and that HS and SPP competitively bind to the crystal, then, as a result, they are incorporated into the crystals. The fact that SPP suppressed the aggregation of CaOx crystals in undiluted urine showed the possibility that SPP might be one of the useful drugs for preventing CaOx urolithiasis.
本研究的目的是检测未稀释尿液系统中戊聚糖多硫酸钠（SPP）的作用，并研究在存在或不存在硫酸乙酰肝素（HS）的情况下其与草酸钙（CaOx）晶体的相对亲和力。在离心过滤尿液（SF）和超滤尿液（UF）中，通过使草酸盐过载至亚稳极限以上来诱导CaOx晶体形成。然后，用乙二胺四乙酸（EDTA）溶解晶体，进行电渗析并冻干。在添加草酸盐之前，将多阴离子HS或SPP添加到UF中。通过醋酸纤维素电泳检测晶体基质中的多阴离子。与UF相比，随着SPP浓度的增加，晶体体积和尺寸受到抑制。扫描电子显微镜（SEM）显示，UF中的晶体明显聚集，而在存在SPP的情况下没有聚集。HS是在SF中草酸盐过载后形成的CaOx晶体中发现的唯一多阴离子。UF中形成的晶体不含任何多阴离子。当将SPP添加到UF中时，SPP会根据其浓度出现在晶体基质中。一旦将生理浓度的HS添加到含有SPP的UF中，在电泳研究中，从晶体中获得的HS和SPP会被阿尔辛蓝强烈染色，其中SPP的染色比HS更强。这些结果表明，SPP与HS一样能强烈结合到CaOx晶体上，并且HS和SPP竞争性地结合到晶体上，进而被纳入晶体中。SPP在未稀释尿液中抑制CaOx晶体聚集这一事实表明，SPP有可能成为预防CaOx尿路结石的有用药物之一。
