Kawamura K, Moriyama M, Nakajima C, Miyazawa K, Suzuki K, Tsugawa R
Department of Urology, Kanazawa Medical University.
Hinyokika Kiyo. 1993 Aug;39(8):695-700.
The inhibitory effects of Takusha on the formation, growth and aggregation of calcium oxalate crystals were estimated by the microplate method and the use of Coulter counter TAII in the diluted urine system and undiluted urine system. In the diluted urine system, the inhibitory effect on aggregation and growth was calculated from the changes in the number and volume of crystals by the seed crystal method. Takusha had a strong inhibitory effect on the aggregation and growth when the concentration was above 10 micrograms/ml. In measuring the metastable limit by the microplate method, Takusha had a mild inhibitory effect on the formation of crystals above the concentration of 1 mg/ml. In the undiluted urine system, after determining the metastable limit, the formation and growth of calcium oxalate crystals which precipitated in response to a load of sodium oxalate were measured. Takusha had a strong inhibitory effect on the formation and growth at the concentration of 0.1-1 mg/ml. Takusha had no glycosaminoglicans in the dimethylmethylene blue assay and had an inhibitory effect on aggregation and growth when its molecular weight was over 10,000. Therefore, Takusha might be useful for preventing stones in recurrent stone formers.
通过微孔板法以及在稀释尿液系统和未稀释尿液系统中使用库尔特计数器TAII，评估了拓株对草酸钙晶体形成、生长和聚集的抑制作用。在稀释尿液系统中，采用晶种法根据晶体数量和体积的变化计算对聚集和生长的抑制作用。当浓度高于10微克/毫升时，拓株对聚集和生长具有强烈的抑制作用。在通过微孔板法测量亚稳极限时，浓度高于1毫克/毫升时，拓株对晶体形成具有轻微的抑制作用。在未稀释尿液系统中，确定亚稳极限后，测量了因草酸钠负荷而沉淀的草酸钙晶体的形成和生长情况。在浓度为0.1 - 1毫克/毫升时，拓株对形成和生长具有强烈的抑制作用。在二甲苯亚甲基蓝测定中，拓株不含糖胺聚糖，当其分子量超过10000时，对聚集和生长具有抑制作用。因此，拓株可能对预防复发性结石患者的结石有用。
