a Department of Biology , College of Basic Sciences, Science and Research Branch, Islamic Azad University , Tehran , Iran.
b Department of Biology , College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University , Varamin-Pishva , Iran.
Ren Fail. 2018 Nov;40(1):298-305. doi: 10.1080/0886022X.2018.1455595.
We investigated and compared the effects of taraxasterol, aqueous extract of T. officinale (AET) aerial part, and potassium citrate (PC) on calcium oxalate (CaOx) crystallization in vitro.
CaOx crystallization was induced by adding sodium oxalate to synthetic urine. Taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL), extract (1, 2, 4 and 8 mg/mL), and PC (100, 150, 200 and 350 mg/mL) were subjected to anti-crystallization activities. The absorbance and %inhibition of nucleation of CaOx crystals were evaluated by spectrophotometer at 2, 4, 6, 8, 10, 20, 30, 40, 50 and 60 min and the number and morphology of crystals were studied by light microscopy after 60 min.
Presence of taraxasterol, extract and PC decreased absorbance in experimental samples compared to control, significantly. The nucleation of crystals is inhibited by taraxasterol, extract, and PC (26-64, 55-63 and 60-70%, respectively). The number of CaOx crystals were decreased in presence of taraxasterol (p < .01), extract (p < .001), and PC (p < .001) in a dose-dependent manner. Presence of taraxasterol, extract, and PC decreased the number of CaCO monohydrate, while increased CaCO dihydrate crystals, significantly. Also, the diameter of CaCO dihydrate crystals was decreased in presence of taraxasterol, extract and PC, significantly.
This research indicated that taraxasterol and extract have anti-crystallization activities and effectiveness of the extract is more potent than taraxasterol. It could be because of another constituent in the extract with the synergistic effect.
研究并比较蒲公英甾醇、蒲公英地上部分水提物（AET）和柠檬酸钾（PC）对体外草酸钙（CaOx）结晶的影响。
通过向合成尿液中添加草酸钠来诱导 CaOx 结晶。蒲公英甾醇（2.5、5、7.5 和 12.5μg/mL）、提取物（1、2、4 和 8mg/mL）和 PC（100、150、200 和 350mg/mL）进行抗结晶活性测试。通过分光光度计在 2、4、6、8、10、20、30、40、50 和 60min 时评估 CaOx 晶体成核的吸光度和抑制率%，并在 60min 后通过光镜研究晶体的数量和形态。
与对照组相比，蒲公英甾醇、提取物和 PC 的存在显著降低了实验样品的吸光度。蒲公英甾醇、提取物和 PC 抑制晶体成核（分别为 26-64%、55-63%和 60-70%）。蒲公英甾醇（p<.01）、提取物（p<.001）和 PC（p<.001）的存在呈剂量依赖性降低了 CaOx 晶体的数量。蒲公英甾醇、提取物和 PC 的存在显著减少了 CaCO 一水合物的数量，同时增加了 CaCO 二水合物晶体的数量。此外，蒲公英甾醇、提取物和 PC 的存在显著降低了 CaCO 二水合物晶体的直径。
本研究表明，蒲公英甾醇和提取物具有抗结晶活性，且提取物的效果比蒲公英甾醇更有效。这可能是因为提取物中存在另一种成分，具有协同作用。
