Boysen M, Borja M, del Moral C, Salazar O, Rubio V
Centro Nacional de Biotecnologia, Universidad Autonoma de Madrid, Spain.
Curr Genet. 1996 Jan;29(2):174-81. doi: 10.1007/BF02221582.
The relatedness of nine isolates of Rhizoctonia solani, belonging to anastomosis group (AG) 4, and one isolate of AG1 was determined by comparative sequence analysis based on direct sequencing of PCR-amplified ribosomal DNA [the internal transcribed spacer (ITS) region and the 5.8 s ribosomal DNA]. The 5.8 s rDNA is completely conserved, but both ITS regions show variation among strains. AG1 was an outgroup based on anastomosis ability and RFLP analyses. Phylogenetic analyses based on the ITS sequences suggest that the analyzed AG4 strains can be divided into three groups that correlate with habitat and virulence.
通过对聚合酶链反应（PCR）扩增的核糖体DNA[内转录间隔区（ITS）和5.8 s核糖体DNA]进行直接测序的比较序列分析，确定了9株属于融合群（AG）4的立枯丝核菌分离株和1株AG1分离株之间的亲缘关系。5.8 s核糖体DNA完全保守，但两个ITS区域在菌株间存在变异。基于融合能力和限制性片段长度多态性（RFLP）分析，AG1是一个外类群。基于ITS序列的系统发育分析表明，所分析的AG4菌株可分为与栖息地和毒力相关的三组。
