Muthumeenakshi S, Mills P R, Brown A E, Seaby D A
Department of Applied Plant Science, Queen's University of Belfast, UK.
Microbiology (Reading). 1994 Apr;140 ( Pt 4):769-77. doi: 10.1099/00221287-140-4-769.
The genetic diversity in Trichoderma harzianum isolates from mushroom compost was assessed using various molecular techniques. Restriction fragment length polymorphism (RFLP) analysis of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) divided the 81 isolates into three major groups, 1, 2 and 3. There was no variation within a group in rDNA, while a low degree of polymorphism was detected in mtDNA. Random amplified polymorphic DNA (RAPD) analysis of 30 randomly chosen isolates, with six primers, in general confirmed the RFLP groups. Nucleotide sequence determination of rDNA internal transcribed spacer (ITS) 1 revealed three distinct ITS types, 1, 2 and 3, possessed by isolates from the respective groups 1, 2 and 3. Based on these molecular data, group 2 isolates, which are aggressive colonizers of mushroom compost, could be clearly distinguished from the isolates belonging to the other two groups.
使用各种分子技术评估了来自蘑菇堆肥的哈茨木霉分离株的遗传多样性。核糖体DNA（rDNA）和线粒体DNA（mtDNA）的限制性片段长度多态性（RFLP）分析将81个分离株分为三个主要组，即1组、2组和3组。rDNA在一个组内没有变异，而在mtDNA中检测到低水平的多态性。用六种引物对30个随机选择的分离株进行随机扩增多态性DNA（RAPD）分析，总体上证实了RFLP分组。rDNA内部转录间隔区（ITS）1的核苷酸序列测定揭示了三种不同的ITS类型，即1型、2型和3型，分别由相应的1组、2组和3组的分离株所拥有。基于这些分子数据，作为蘑菇堆肥积极定殖者的2组分离株可以与属于其他两组的分离株明显区分开来。
