Velasco A, Caminos E, Vecino E, Lara J M, Aijón J
Departamento de Biologia Cellular, Universidad de Salamanca, Spain.
Brain Res. 1995 Dec 24;705(1-2):315-24. doi: 10.1016/0006-8993(95)01204-4.
We have studied the microglial cells in the normal and regenerating visual pathways of Tinca tinca (Cyprinid, Teleost) by using the lectin from Lycopersicum esculentum (tomato), which, in our case, has been demonstrated as a specific marker for teleost microglia. In the normal fish, there are tomato lectin positive microglial cells in the retina, optic nerve, and optic tectum. Following optic nerve crush, we observed a more extensive labeling of the microglia in the crushed optic nerve and in the contralateral optic tectum affecting the stratum opticum and stratum fibrosum et griseum superficiale. In both cases, there was an increase of rounded and less ramified microglial cells, and granular cells. This response of a more extensive labeling of microglial cells increases to a maximum at 2-3 weeks after the crush; the density of labeled microglial cells decreases after 3 months after crushing. However, in the retina no changes were observed after optic nerve crush. These results suggest that the microglial cells could play an important role in regeneration of fish optic pathway, as other neuroglial cells do.
我们利用来自番茄（Lycopersicum esculentum）的凝集素，研究了丁鱥（Tinca tinca，鲤科鱼类，硬骨鱼）正常及再生视觉通路中的小胶质细胞。在我们的研究中，这种凝集素已被证明是硬骨鱼小胶质细胞的特异性标记物。在正常鱼类中，视网膜、视神经和视顶盖存在番茄凝集素阳性的小胶质细胞。视神经挤压伤后，我们观察到挤压侧视神经和对侧视顶盖中小胶质细胞的标记范围更广，影响到视层和浅层纤维灰质层。在这两种情况下，圆形且分支较少的小胶质细胞以及颗粒细胞数量均增加。小胶质细胞标记范围更广的这种反应在挤压伤后2 - 3周达到最大值；挤压伤3个月后，标记的小胶质细胞密度降低。然而，视神经挤压伤后视网膜未观察到变化。这些结果表明，小胶质细胞可能像其他神经胶质细胞一样，在鱼类视觉通路再生中发挥重要作用。
