Jimeno D, Velasco A, Lillo C, Lara J M, Aijón J
Instituto de Neurociencias de Castilla y León (INCyL), Departamento de Biología Celular y Patología, Facultad de Medicina, Universidad de Salamanca, Avda. Campo Charro s/n, E-37007, Salamanca, Spain.
Brain Res. 1999 Jan 16;816(1):175-89. doi: 10.1016/s0006-8993(98)01170-6.
We studied the glial response after inducing a lesion in the zone of the peripheral retina of tench, where there is proliferative neuroepithelium. In the retina and optic nerve, the microglial response was analysed with tomato lectin and the macroglial response with antibodies against GFAP and S-100. In lesioned retinas, there was a temporal-spatial distribution pattern of microglia. One day after lesion, primitive ramified cells appeared in the nerve fibre layer. These cells appeared progressively from the vitreal to the scleral layers until day 7 when cells appeared in all layers, with the exception of the outer plexiform layer. From this point, labelling decreased. In the optic nerve, 3 days after lesion, an increase in the number of microglial cells was observed, first in the nerve folds and from day 15 in specific areas of the optic nerve. In the central retina, in the optic nerve head and within the optic nerve itself, the appearance of microglial cells, after the lesion, near the blood vessels, could indicate a vascular origin of microglia, as has been proposed by many authors. However, we cannot discount the idea that some of the reactive microglial cells arise by proliferation of the microglia existing in the normal state. Using GFAP and S-100 antibodies, no important changes in the retina were observed, however in the optic nerve there was response to the lesion. Thus, the macroglial cells appeared to be involved in reorganisation of the optic nerve axons after lesion.
我们研究了在具有增殖性神经上皮的丁鲷外周视网膜区域诱导损伤后的胶质细胞反应。在视网膜和视神经中，用番茄凝集素分析小胶质细胞反应，用抗GFAP和S - 100抗体分析大胶质细胞反应。在受损视网膜中，小胶质细胞呈现出时空分布模式。损伤后一天，原始分支细胞出现在神经纤维层。这些细胞从玻璃体层向巩膜层逐渐出现，直到第7天所有层中都出现细胞，但外网状层除外。从这一点开始，标记减少。在视神经中，损伤后3天，观察到小胶质细胞数量增加，首先出现在神经褶皱处，从第15天开始出现在视神经的特定区域。在中央视网膜、视神经乳头和视神经本身内，损伤后靠近血管处出现小胶质细胞，这可能表明小胶质细胞起源于血管，正如许多作者所提出的那样。然而，我们不能排除这样的观点，即一些反应性小胶质细胞是由正常状态下存在的小胶质细胞增殖产生的。使用GFAP和S - 100抗体，在视网膜中未观察到重要变化，然而在视神经中对损伤有反应。因此，大胶质细胞似乎参与了损伤后视神经轴突的重组。
