Velasco A, Briñón J G, Caminos E, Lara J M, Aijón J
Departamento de Biología Celular y Patología, Universidad de Salamanca, Spain.
Brain Res Bull. 1997;43(3):327-36. doi: 10.1016/s0361-9230(97)00014-2.
Glial cells in the normal and regenerating visual pathways of Tinca tinca (Cyprinid, Teleost) were studied by labelling with anti-S-100 antibody. In normal fish, S-100-positive bipolar cells were found in the optic nerve, optic tract, and in the diencephalic visual pathways. After crushing the left optic nerve, the distribution and the number of S-100-immunoreactive cells were modified. In the injured nerve, 7 to 15 days after crushing no immunoreactive cell bodies were found in the crushed area, but a greater number of S-100-positive cells were found on both sides of the injured area. Sixty days after crushing, positive cells penetrating the crushed area were observed; the normal pattern was almost restored 200 days after crushing. In the diencephalon, 25 days after crushing, the number of S-100-positive cells increased remarkably and the most intense immunostaining of glial processes was observed 60 days after crushing. The density of S-100-labelled cells decreased after 4 months postcrushing. However, in the optic tectum no changes were observed. The increase of glial cells in the lesioned visual pathway suggests that they could play an important role in axonal regeneration after crushing.
通过抗S-100抗体标记，对丁鱥（鲤科鱼类，硬骨鱼纲）正常和再生视觉通路中的神经胶质细胞进行了研究。在正常鱼类中，视神经、视束和间脑视觉通路中发现了S-100阳性双极细胞。在左侧视神经挤压伤后，S-100免疫反应性细胞的分布和数量发生了改变。在受伤神经中，挤压伤后7至15天，挤压区域未发现免疫反应性细胞体，但在受伤区域两侧发现了更多的S-100阳性细胞。挤压伤60天后，观察到有阳性细胞穿透挤压区域；挤压伤200天后，正常模式几乎恢复。在间脑中，挤压伤25天后，S-100阳性细胞数量显著增加，挤压伤60天后观察到神经胶质细胞突起的免疫染色最为强烈。挤压伤4个月后，S-100标记细胞的密度降低。然而，在视顶盖未观察到变化。损伤视觉通路中神经胶质细胞的增加表明它们可能在挤压伤后的轴突再生中发挥重要作用。
