He Y L, Ding Y F, Long Y Q
Department of Food Science and Technology, Tianjin Institute of Light Industry, Tianjin, People's Republic of China.
Int J Syst Bacteriol. 1991 Apr;41(2):306-9. doi: 10.1099/00207713-41-2-306.
Two cellulolytic clostridia, one thermophilic and the other mesophilic, were isolated and characterized. Cells of the thermophile are gram-negative rods that are motile with lophotrichous flagella and spherical terminal endospores which swell the cells. The optimum growth temperature is 55 to 60 degrees C, with a range of 40 to 65 degrees C. The deoxyribonucleic acid composition is 35 mol% G + C. The name Clostridium cellulosi sp. nov. is proposed. The type strain is AS 1.1777. Cells of the mesophile are gram negative and motile with peritrichous flagella and terminal oval or spherical spores which swell the cells. The deoxyribonucleic acid composition is 34 mol% G + C. The name Clostridium cellulofermentans sp. nov. is proposed. The type strain is AS 1.1775. Both C. cellulosi AS 1.1777 and C. cellulofermentans AS 1.1775 are deposited in the China Committee for Culture Collection of Microorganisms, Institute of Microbiology, Academia Sinica, Beijing, People's Republic of China.
分离并鉴定了两种纤维素分解梭菌，一种嗜热，另一种嗜温。嗜热菌的细胞为革兰氏阴性杆菌，具束状鞭毛，能运动，芽孢位于细胞末端呈球形，可使细胞膨大。最适生长温度为55至60℃，生长温度范围为40至65℃。脱氧核糖核酸组成中G + C含量为35摩尔%。建议命名为纤维素梭菌（Clostridium cellulosi sp. nov.）。模式菌株为AS 1.1777。嗜温菌的细胞为革兰氏阴性，具周生鞭毛，能运动，芽孢位于细胞末端呈椭圆形或球形，可使细胞膨大。脱氧核糖核酸组成中G + C含量为34摩尔%。建议命名为纤维发酵梭菌（Clostridium cellulofermentans sp. nov.）。模式菌株为AS 1.1775。纤维素梭菌AS 1.1777和纤维发酵梭菌AS 1.1775均保藏于中华人民共和国北京市中国科学院微生物研究所中国微生物菌种保藏管理委员会。
