James K J, Sherlock I R
Chemistry Department, Cork RTC, Ireland.
Biomed Chromatogr. 1996 Jan-Feb;10(1):46-7. doi: 10.1002/(SICI)1099-0801(199601)10:1<46::AID-BMC551>3.0.CO;2-7.
A sensitive HPLC analysis has been developed for the determination of anatoxin-a in cyanobacterial bloom material and in raw and treated waters. Efficient extraction of this toxin from its matrix can be achieved using solid-phase extraction with a weak cation exchanger. HPLC analysis with fluorimetric detection (lambda ex 470 nm, lambda em 530 nm) was possible after derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). This new analytical protocol permits the detection of anatoxin-a at low concentrations (0.1 micrograms/L) and gave a good linear calibration (1-20 micrograms/L, r = 0.997).
已开发出一种灵敏的高效液相色谱分析法，用于测定蓝藻水华中以及原水和处理后水中的类毒素-a。使用弱阳离子交换剂进行固相萃取，可从其基质中有效提取该毒素。用4-氟-7-硝基-2,1,3-苯并恶二唑（NBD-F）衍生化后，可进行带荧光检测（激发波长470 nm，发射波长530 nm）的高效液相色谱分析。这种新的分析方法能够检测低浓度（0.1微克/升）的类毒素-a，并给出良好的线性校准（1-20微克/升，r = 0.997）。
