Truman P, Lake R J
Institute of Environmental Science and Research Limited, Communicable Disease Centre, Porirua, New Zealand.
J AOAC Int. 1996 Sep-Oct;79(5):1130-3.
A neuroblastoma cell culture assay was used to analyze shellfish extracts for presence of paralytic shellfish poisoning toxins (saxitoxins). Results were compared with mouse bioassays performed as part of a screening program for shellfish toxins in New Zealand. Twenty-nine samples gave negative results in both assays. Fifty-seven samples gave positive results in at least one assay. The correlation between the assays for saxitoxin equivalent levels in shellfish was 0.867. In spiking studies on shellfish extracts, the neuroblastoma assay showed a good response to added saxitoxin. Although these results support use of the neuroblastoma assay as a screening procedure for shellfish toxicity, results close to regulatory limits should be confirmed by mouse bioassay.
采用神经母细胞瘤细胞培养试验分析贝类提取物中是否存在麻痹性贝类中毒毒素（石房蛤毒素）。将结果与作为新西兰贝类毒素筛查项目一部分所进行的小鼠生物测定结果进行比较。29个样本在两种测定中均呈阴性结果。57个样本在至少一种测定中呈阳性结果。贝类中石房蛤毒素等效水平的两种测定之间的相关性为0.867。在对贝类提取物的加标研究中，神经母细胞瘤试验对添加的石房蛤毒素显示出良好反应。尽管这些结果支持将神经母细胞瘤试验用作贝类毒性的筛查程序，但接近监管限值的结果应通过小鼠生物测定加以确认。
