Kang M S, Lee H J, Lee J H, Ku J L, Lee K P, Kelley M J, Won Y J, Kim S T, Park J G
Laboratory of Cell Biology, Cancer Research Center and Cancer Research Institute, Seoul National University College of Medicine, Korea.
Int J Cancer. 1996 Sep 17;67(6):898-902. doi: 10.1002/(SICI)1097-0215(19960917)67:6<898::AID-IJC22>3.0.CO;2-X.
It has been known that the incidence of p53 mutation is very rare in HBX-positive primary human hepatocellular carcinoma (HCC) tissues. The frequency of p53 mutation, however, in established cell lines with integrated HBV DNA and/or HBX has not been well studied. To know p53 mutational frequency, and to investigate whether the presence of HBX DNA sequence correlates with the absence of p53 mutation in the established HCC cell lines, we studied the p53 mutation and the presence of HBX sequence in 12 recently characterized HCC cell lines. As a result, all 12 (100%) lines showed mutation in the p53 gene. Three (25%) cell lines had transversion of codon 215 while no mutation of codon 249 was found. In contrast with previous reports, although HBX DNA was present in 11 cell lines, p53 mutation had occurred, indicating that the presence of HBX viral DNA does not correlate with a lack of p53 mutation in established HCC cell lines. Our results suggest that the frequency of p53 mutation is extremely high even in HBX DNA positive HCC cell lines.
已知在HBX阳性的原发性人类肝细胞癌（HCC）组织中，p53突变的发生率非常低。然而，在整合了HBV DNA和/或HBX的已建立细胞系中，p53突变的频率尚未得到充分研究。为了了解p53突变频率，并研究HBX DNA序列的存在是否与已建立的HCC细胞系中p53突变的缺失相关，我们研究了12个最近鉴定的HCC细胞系中的p53突变和HBX序列的存在情况。结果，所有12个（100%）细胞系均显示p53基因发生突变。三个（25%）细胞系发生了215密码子的颠换，而未发现249密码子的突变。与先前的报道相反，尽管11个细胞系中存在HBX DNA，但p53突变仍然发生，这表明HBX病毒DNA的存在与已建立的HCC细胞系中p53突变的缺失无关。我们的结果表明，即使在HBX DNA阳性的HCC细胞系中，p53突变的频率也极高。
