枯草芽孢杆菌嘧啶调节蛋白PyrR在体内通过与pyr mRNA的三个位点结合发挥作用的证据。
Evidence that the Bacillus subtilis pyrimidine regulatory protein PyrR acts by binding to pyr mRNA at three sites in vivo.
作者信息
Lu Y, Switzer R L
机构信息
Department of Biochemistry, University of Illinois, Urbana 61801, USA.
出版信息
J Bacteriol. 1996 Oct;178(19):5806-9. doi: 10.1128/jb.178.19.5806-5809.1996.
Abstract
The Bacillus subtilis pyr operon is regulated by a transcriptional attenuation mechanism that requires the PyrR regulatory protein. Multicopy plasmids that could be transcribed to yield segments of RNA from the attenuation regions of the pyr operon induced derepression of chromosomal pyr genes, whereas plasmids that could not yield pyr RNA did not. We conclude that pyr RNA acts by titrating the PyrR protein and preventing it from regulating pyr attenuation.
摘要
枯草芽孢杆菌的pyr操纵子受转录衰减机制调控，该机制需要PyrR调节蛋白。能够转录产生来自pyr操纵子衰减区域的RNA片段的多拷贝质粒可诱导染色体上pyr基因的去阻遏，而不能产生pyr RNA的质粒则不会。我们得出结论，pyr RNA通过滴定PyrR蛋白并阻止其调节pyr衰减而起作用。
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