Satpathy G, Mohanty S, Nayak N
Dept. of Microbiology, Dr. R. P. Centre for Ophthalmic Sciences, A.I.I.M.S, New Delhi.
Indian J Ophthalmol. 1996 Mar;44(1):19-21.
An epidemic of acute haemorrhagic conjunctivitis affecting persons of all ages and both sexes occurred in Delhi and surrounding areas during the monsoon season of 1994. The symptoms lasted on an average for 4-5 days. In some of the patients corneal involvement was observed. Conjunctival swabs from the affected patients were processed for viral antigen detection, virus isolation and bacterial culture and sensitivity. Viral antigen was detected in 62% (31/50) of the smears tested by indirect immunofluorescence assay. In 22 (44%) of the specimens Coxackie A 24 (Cox A 24) virus antigen and in 9 (18%) of the specimens Entero Virus 70 (EV 70) antigen were detected. In confluent monolayers of Hep 2 cells cytopathic virus was isolated in 10 (30.30%) of the 33 specimens processed. The isolated viruses were identified as either Cox A 24 (7 isolates) or EV 70 (3 isolates) using indirect immunofluorescence assay. Super added bacterial infection was observed in 33% (89/270) of the cases, Staphylococcus albus being the predominant bacteria isolated.
1994年季风季节期间，德里及其周边地区爆发了急性出血性结膜炎疫情，各年龄段和性别的人都受到影响。症状平均持续4至5天。部分患者出现角膜受累情况。对受影响患者的结膜拭子进行处理，以检测病毒抗原、分离病毒以及进行细菌培养和药敏试验。通过间接免疫荧光法检测的涂片样本中，62%（31/50）检测到病毒抗原。在22份（44%）标本中检测到柯萨奇A24（Cox A 24）病毒抗原，在9份（18%）标本中检测到肠道病毒70（EV 70）抗原。在处理的33份标本中，有10份（30.30%）在Hep 2细胞融合单层中分离出具有细胞病变效应的病毒。使用间接免疫荧光法将分离出的病毒鉴定为柯萨奇A24（7株）或肠道病毒70（3株）。33%（89/270）的病例观察到继发细菌感染，分离出的主要细菌为白色葡萄球菌。
