Suzuki K, Onodera S, Matsuno T, Kaneda K, Nishihira J
Department of Orthopedic Surgery, Hokkaido University School of Medicine, Japan.
Biochem Mol Biol Int. 1996 Jun;39(3):529-37. doi: 10.1080/15216549600201581.
Endosteal bone resorption often occurs around loosened total joint replacements. In the process of the loosening, macrophages play an important role by releasing cytokines such as interleukin-1, tumor necrosis factor-alpha and prostaglandin E2. In this study, we investigated the involvement of macrophage migration inhibitory factor (MIF) in the pathological state of the loosening of a total hip replacement. Interface membranes were harvested from bone-cement or bone-implant interfaces of two patients during revision hip surgeries. The tissues were immunohistochemically examined with a polyclonal antibody against human recombinant MIF. This study detected MIF in the cytoplasm of the macrophages in all the tissues tested, though it was not detected in that of interstitial cells. The expression of MIF mRNA in the membrane was also examined by reverse transcription polymerase chain reaction, which demonstrated that expression of the MIF mRNA in the interface membranes was higher than that of the normal synovium. Considering these results, it is suggested that MIF is one of important cytokines mediating the inflammatory process during loosening of total joint replacements.
骨内膜骨吸收常发生在松动的全关节置换周围。在松动过程中，巨噬细胞通过释放白细胞介素 -1、肿瘤坏死因子 -α 和前列腺素 E2 等细胞因子发挥重要作用。在本研究中，我们调查了巨噬细胞迁移抑制因子（MIF）在全髋关节置换松动病理状态中的作用。在翻修髋关节手术期间，从两名患者的骨水泥或骨植入物界面采集界面膜。用抗人重组 MIF 的多克隆抗体对组织进行免疫组织化学检查。本研究在所有测试组织的巨噬细胞胞质中检测到 MIF，但在间质细胞中未检测到。还通过逆转录聚合酶链反应检测了膜中 MIF mRNA 的表达，结果表明界面膜中 MIF mRNA 的表达高于正常滑膜。考虑到这些结果，提示 MIF 是介导全关节置换松动期间炎症过程的重要细胞因子之一。
