Husson A, Quillard M, Fairand A, Chedeville A, Lavoinne A
Institut Fédératif de Recherches Multidisciplinaires sur les PeptidesNo. 23 (IFRMP), U.F.R. Médecine-Pharmacie de Rouen, Saint-Etienne-du-Rouvray Cedex, France.
FEBS Lett. 1996 Oct 7;394(3):353-5. doi: 10.1016/0014-5793(96)00985-4.
The mechanism of action of hydration state was studied on beta-actin gene expression in isolated hepatocytes. Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarity increased and hyperosmolarity decreased the beta-actin mRNA level through a corresponding modulation of the rate of the gene transcription. Glutamine, which is known to induce cell swelling, also increased the beta-actin mRNA level in a dose-dependent manner and induced a stimulation of the beta-actin gene transcription. Thus, cell hydration state regulates gene expression in the liver through a transcriptional mechanism.
研究了水合状态对分离的肝细胞中β-肌动蛋白基因表达的作用机制。通过Northern印迹分析和转录分析得出的结果表明，低渗增加而高渗降低β-肌动蛋白mRNA水平，这是通过对基因转录速率的相应调节实现的。已知能诱导细胞肿胀的谷氨酰胺也以剂量依赖性方式增加β-肌动蛋白mRNA水平，并诱导β-肌动蛋白基因转录的刺激。因此，细胞水合状态通过转录机制调节肝脏中的基因表达。
