用于鉴定百日咳博德特氏菌和副百日咳博德特氏菌的聚合酶链反应
Polymerase chain reaction for the identification of Bordetella pertussis and Bordetella parapertussis.
作者信息
Stefanelli P, Giuliano M, Bottone M, Spigaglia P, Mastrantonio P
机构信息
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Roma, Italy.
出版信息
Diagn Microbiol Infect Dis. 1996 Apr;24(4):197-200. doi: 10.1016/0732-8893(96)00064-8.
The polymerase chain reaction (PCR) for the detection of Bordetella pertussis and Bordetella parapertussis DNA in clinical samples was well documented by recent studies. Different regions in Bordetella pertussis DNA have been successfully used as targets for this method by various authors. In this work we report the usefulness of the PCR assay also for speciating Bordetellae isolates in those cases where the biochemical and serological tests gave inconclusive results.
近期研究充分证明了利用聚合酶链反应（PCR）检测临床样本中百日咳博德特氏菌和副百日咳博德特氏菌DNA的方法。不同作者已成功将百日咳博德特氏菌DNA的不同区域用作该方法的靶标。在本研究中，我们报告了在生化和血清学检测结果不明确的情况下，PCR检测法对于博德特氏菌分离株进行菌种鉴定的实用性。
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