Hong T, Ndamukong J, Millett W, Kish A, Win K K, Choi Y J
Department of Pathology, Bronx-Lebanon Hospital Center, New York, USA.
Diagn Microbiol Infect Dis. 1996 May;25(1):21-5. doi: 10.1016/0732-8893(96)00062-4.
The Etest was applied directly to 100 Gram-positive bacterial strains from blood cultures to measure their minimum inhibitory concentration (MIC). Results showed 100% concordance of MIC data between a direct Etest method and the standard Etest method for Streptococcus pneumoniae, beta-hemolytic streptococci, and viridans group streptococci. In addition, direct Etest for Staphylococcus aureus, coagulase negative staphylococci, Enterococcus faecium, and Enterococcus faecalis showed 83 to 100% correlation with standard Etests. These data indicate that the Etest is useful to obtain MIC data on Gram-positive cocci (especially streptococci) directly from positive blood cultures. The advantages of a direct Etest are two fold: MIC results can be obtained 24 hours earlier than standard methods and a more representative population of the bacterial isolate is tested.
将Etest直接应用于100株来自血培养的革兰氏阳性菌，以测定其最低抑菌浓度（MIC）。结果显示，对于肺炎链球菌、β溶血性链球菌和草绿色链球菌，直接Etest法与标准Etest法之间的MIC数据一致性为100%。此外，金黄色葡萄球菌、凝固酶阴性葡萄球菌、粪肠球菌和屎肠球菌的直接Etest与标准Etest的相关性为83%至100%。这些数据表明，Etest可直接从阳性血培养中获得革兰氏阳性球菌（尤其是链球菌）的MIC数据。直接Etest的优点有两个：MIC结果比标准方法可提前24小时获得，且检测的细菌分离株群体更具代表性。
