Bacigalupo M A, Bazzini P, Farina L, Ius A
Istituto di Chimica degli Ormoni CNR, Milano, Italy.
Eur J Clin Chem Clin Biochem. 1996 Jun;34(6):503-5.
A time-resolved immunofluorometric assay of the IgG specific for Toxoplasma gondii has been compared with two commercial methods, one automated enzyme linked fluorescent assay (VIDAS) and one automated colorimetric enzyme immunoassay (BEIA). The coefficients of variation were 3.4% and 7.4% within-assay (n = 12), and 9.2% and 8.1% between-assay (n = 10) for two sera at low and high concentrations of specific IgG. The regression lines obtained with 96 samples were y = 1.04x + 2.1 and y = 0.98x - 1.1. We also compared a time-resolved capture fluoroimmunoassay for Toxoplasma-specific IgM with a commercial immunoenzymatic assay (BEIA TOXO-M). The sensitivity and specificity were 100%, calculated from assays of 78 samples.
已将一种针对弓形虫IgG的时间分辨免疫荧光测定法与两种商业方法进行了比较，一种是自动酶联荧光测定法（VIDAS），另一种是自动比色酶免疫测定法（BEIA）。对于两种低浓度和高浓度特异性IgG血清，批内变异系数分别为3.4%和7.4%（n = 12），批间变异系数分别为9.2%和8.1%（n = 10）。用96个样本得到的回归线分别为y = 1.04x + 2.1和y = 0.98x - 1.1。我们还将一种针对弓形虫特异性IgM的时间分辨捕获荧光免疫测定法与一种商业免疫酶测定法（BEIA TOXO-M）进行了比较。根据对78个样本的检测计算，灵敏度和特异性均为100%。
