弓形虫免疫球蛋白G（IgG）和免疫球蛋白M（IgM）抗体检测：四种商用免疫分析系统的评估
Detection of immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii: evaluation of four commercial immunoassay systems.
作者信息
Hofgärtner W T, Swanzy S R, Bacina R M, Condon J, Gupta M, Matlock P E, Bergeron D L, Plorde J J, Fritsche T R
机构信息
Department of Laboratory Medicine, University of Washington, Seattle, USA.
出版信息
J Clin Microbiol. 1997 Dec;35(12):3313-5. doi: 10.1128/jcm.35.12.3313-3315.1997.
Abstract
A comparative evaluation of the following commercial immunoassays for the determination of antibodies to Toxoplasma gondii was performed: Behring Diagnostics OPUS Toxo G and Toxo M, Abbott Diagnostics IMX Toxo-IgG 2.0 and Toxo-IgM, Sanofi Diagnostics Pasteur Platelia Toxo IgG and Toxo IgM, and bioMérieux Vitek VIDAS Toxo IgG and IgM. Of 676 specimens that were tested for Toxoplasma-specific immunoglobulin G (IgG) antibodies, 26% were reactive by all methods while 8% displayed some discrepancy. Of 718 specimens that were tested for Toxoplasma-specific IgM antibodies, 3% were reactive by all methods while 10% displayed some discrepancy. Analysis of discrepant specimens revealed performance shortcomings with all IgM-specific assays. The impact of such shortcomings is magnified in a population with a low prevalence of toxoplasmosis.
摘要
对以下用于检测弓形虫抗体的商用免疫测定法进行了比较评估
贝林诊断公司的OPUS Toxo G和Toxo M、雅培诊断公司的IMX Toxo-IgG 2.0和Toxo-IgM、赛诺菲诊断公司巴斯德分公司的Platelia Toxo IgG和Toxo IgM以及生物梅里埃公司的Vitek VIDAS Toxo IgG和IgM。在676份检测弓形虫特异性免疫球蛋白G（IgG）抗体的标本中，26%的标本在所有方法下均呈阳性反应，而8%的标本存在一些差异。在718份检测弓形虫特异性IgM抗体的标本中，3%的标本在所有方法下均呈阳性反应，而10%的标本存在一些差异。对有差异的标本进行分析发现，所有IgM特异性检测方法均存在性能缺陷。在弓形虫病患病率较低的人群中，这些缺陷的影响会被放大。
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