Mita H, Yasueda H, Ishii T, Akiyama K
Clinical Research Center, National Sagamihara Hospital, Kanagawa, Japan.
Allergy. 1995 Dec;50(12):952-8. doi: 10.1111/j.1398-9995.1995.tb02506.x.
Experiments were done to clarify the mechanisms associated with releasability of histamine. First, washed leukocytes from 23 asthmatic patients sensitive to mite allergen were challenged with Der p 1, a major allergen isolated from Dermatophagoides pteronyssinus, or anti-IgE. A significant correlation was observed between the ratio of Der p 1-specific IgE titer to total IgE level (S/T) in the patient's plasma and either the reactivity (maximal percentage of histamine release; rs = 0.514, P = 0.016, n = 23) or the sensitivity (the minimum allergen concentration required to achieve 25% histamine release; rs = -0.790, P = 0.0002) to Der p 1. Additionally, the reactivity to Der p 1 was significantly correlated with that to anti-IgE (rs = 0.690, P = 0.0012), indicating that an intrinsic cellular property may be one of the contributing factors in immunologic histamine release. In a second series of experiments, sinus mast cells were passively sensitized with immunoglobulins prepared from the patient's plasma. A statistically significant correlation was found between either the reactivity or the sensitivity to Der p 1 and S/T, thus indicating that S/T is an indicator of the releasability of histamine. When basophils or mast cells were passively sensitized with mouse IgE and subsequently stimulated with antimouse IgE, the reactivity to antihuman IgE was significantly correlated with that to antimouse IgE (rs = 0.966, P = 0.0023, n = 11). These observations suggest that an intrinsic cellular property regulates reactivity in immunologic histamine release. Taken together, our results suggest that an intrinsic cellular property, as well as specific IgE antibody levels on the cell surface, is an important factor in determining histamine release in response to IgE-dependent activation.
进行了实验以阐明与组胺释放相关的机制。首先，用从粉尘螨中分离出的主要变应原Der p 1或抗IgE对23名对螨变应原敏感的哮喘患者的洗涤白细胞进行刺激。在患者血浆中Der p 1特异性IgE滴度与总IgE水平之比（S/T）与对Der p 1的反应性（组胺释放的最大百分比；rs = 0.514，P = 0.016，n = 23）或敏感性（达到25%组胺释放所需的最低变应原浓度；rs = -0.790，P = 0.0002）之间观察到显著相关性。此外，对Der p 1的反应性与对抗IgE的反应性显著相关（rs = 0.690，P = 0.0012），表明内在细胞特性可能是免疫性组胺释放的促成因素之一。在第二系列实验中，用从患者血浆制备的免疫球蛋白对鼻窦肥大细胞进行被动致敏。在对Der p 1的反应性或敏感性与S/T之间发现了统计学上的显著相关性，因此表明S/T是组胺释放能力的一个指标。当用小鼠IgE对嗜碱性粒细胞或肥大细胞进行被动致敏，随后用抗小鼠IgE刺激时，对抗人IgE的反应性与对抗小鼠IgE的反应性显著相关（rs = 0.966，P = 0.0023，n = 11）。这些观察结果表明内在细胞特性调节免疫性组胺释放中的反应性。综上所述，我们的结果表明内在细胞特性以及细胞表面的特异性IgE抗体水平是决定IgE依赖性激活后组胺释放的重要因素。
