Weyer A, Le Mao J, Etievant M, David B, Guinnepain M T, Saint-Remy J M
Service d'Allergologie, Institut Pasteur, Paris, France.
Clin Exp Allergy. 1995 Oct;25(10):935-41. doi: 10.1111/j.1365-2222.1995.tb00395.x.
Anti-idiotypic antibodies (anti-Ids) to specific IgE antibodies are formed spontaneously during an anti-allergen immune response and can be induced by immunotherapy. Although anti-Ids can down-regulate the production of IgE antibodies, at least in experimental models, their possible role in the modulation of target cell reactivity remains ill-defined.
The capacity of human anti-Ids to modulate the release of histamine was examined in an in vitro system of human basophil degranulation. Anti-Ids were prepared from the serum of six Dermatophagoides pteronyssinus (Dp)-hypersensitive patients suffering from atopic dermatitis and who had never been desensitized. Basophils were obtained from the blood of atopic donors. The extent of histamine release was determined using a fluorometric assay.
We show that: anti-Ids trigger the release of histamine in an allergen-specific, dose- and IgE-dependent manner; the release is not due to the presence of allergen and/or anti-IgE antibodies; and that the degranulating activity can be removed by absorption with affinity-purified anti-Dp antibodies of the corresponding patient.
These results indicate that spontaneously produced human anti-Ids can modulate the reactivity of human basophils.
针对特异性IgE抗体的抗独特型抗体（抗Id）在抗变应原免疫反应过程中可自发形成，也可通过免疫疗法诱导产生。尽管抗Id至少在实验模型中可下调IgE抗体的产生，但其在调节靶细胞反应性方面的可能作用仍不明确。
在人嗜碱性粒细胞脱颗粒的体外系统中检测人抗Id调节组胺释放的能力。抗Id取自6名患有特应性皮炎且从未接受过脱敏治疗的对屋尘螨（Dp）过敏的患者的血清。嗜碱性粒细胞取自特应性供体的血液。使用荧光测定法确定组胺释放程度。
我们发现：抗Id以变应原特异性、剂量和IgE依赖性方式触发组胺释放；释放并非由于变应原和/或抗IgE抗体的存在；并且脱颗粒活性可通过用相应患者的亲和纯化抗Dp抗体吸收而消除。
这些结果表明，自发产生的人抗Id可调节人嗜碱性粒细胞的反应性。
