Bouzyk M, Evans C, Cullin L, Cox S, Warne D, Nyberg K, Bryant S P, Spurr N K
Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, ENG.
Ann Hum Genet. 1996 Jan;60(1):73-7. doi: 10.1111/j.1469-1809.1996.tb01174.x.
Screening of single human chromosome plasmid libraries using a digoxygenin labelled (AAAT)15 oligonucleotide probe led to the identification of several positive clones. DNA sequence analysis of these was carried out and showed the presence of a number of simple DNA repeats. Oligonucleotide primers were designed from the sequences flanking these repeats and tested in PCR amplification reactions of human genomic DNA. Three of the markers tested were shown to be polymorphic with heterozygosities ranging from 40% to 69%. The markers were assigned to chromosomes using a panel of monochromosomal somatic cell hybrids combined with linkage analysis using DNA from the CEPH panel of families. The markers designated (AAAT)11, (AAAT)12 and (CA)19 were thus assigned to chromosomes 3, 21 and 20 respectively.
使用地高辛标记的(AAAT)15寡核苷酸探针筛选单个人类染色体质粒文库，鉴定出了几个阳性克隆。对这些克隆进行了DNA序列分析，结果显示存在许多简单的DNA重复序列。根据这些重复序列两侧的序列设计了寡核苷酸引物，并在人类基因组DNA的PCR扩增反应中进行了测试。所测试的三个标记显示为多态性，杂合度范围为40%至69%。使用一组单染色体体细胞杂种，并结合来自CEPH家族面板的DNA进行连锁分析，将这些标记定位到染色体上。因此，标记(AAAT)11、(AAAT)12和(CA)19分别被定位到3号、21号和20号染色体上。
