Do Nascimento N, Seebart C S, Francis B, Rogero J R, Kaiser I I
IPEN-CNEN/SP São Paulo, Brazil.
Toxicon. 1996 Jan;34(1):123-31. doi: 10.1016/0041-0101(95)00111-5.
Irradiation of crotoxin and its subunits with 2000 Gy of gamma-rays from 60Co source leads to aggregation and generation of lower mol. wt breakdown products. Aggregates separated by gel filtration retain at least part of their higher-ordered structure, based on their reactivity with monoclonal antibodies known to react with conformational epitopes in native crotoxin. These same aggregates can serve as antigens to raise antisera that cross-react and neutralize crotoxin. Compared with native crotoxin, aggregates appear less myotoxic, are largely devoid of phospholipase activity, and are virtually non-toxic in mice. These results indicate that irradiation of toxic proteins can promote significant detoxification, but still retain many of the original antigenic and immunological properties of native crotoxin.
用来自⁶⁰Co源的2000戈瑞γ射线照射响尾蛇毒素及其亚基会导致聚集，并产生低分子量的降解产物。通过凝胶过滤分离的聚集体基于其与已知可与天然响尾蛇毒素中构象表位发生反应的单克隆抗体的反应性，保留了至少部分其高级结构。这些相同的聚集体可作为抗原引发交叉反应并中和响尾蛇毒素的抗血清。与天然响尾蛇毒素相比，聚集体的肌毒性似乎较小，基本没有磷脂酶活性，并且在小鼠中几乎无毒。这些结果表明，对有毒蛋白质进行照射可促进显著的解毒作用，但仍保留天然响尾蛇毒素的许多原始抗原和免疫特性。
