Kaiser I I, Aird S D
Department of Molecular Biology, University of Wyoming, Laramie 82071.
Toxicon. 1987;25(10):1113-20. doi: 10.1016/0041-0101(87)90268-6.
A major protein toxin from the venom of Crotalus vegrandis was examined by gel filtration, anion-exchange chromatography, and SDS polyacrylamide gel electrophoresis. The toxin was separated into several isoforms by ion-exchange chromatography and spontaneously dissociated into free acidic and basic subunits, mimicking the behavior of crotoxin. Rabbit antisera raised against crotoxin reacted strongly in enzyme-linked immunosorbent assays with the intact C. vegrandis toxin isoforms and their basic subunits, and formed precipitin lines of identity with intact crotoxin in double immunodiffusion gels. These results indicate that vegrandis toxin is strongly homologous with crotoxin from the venom of Crotalus durissus terrificus.
通过凝胶过滤、阴离子交换色谱法和SDS聚丙烯酰胺凝胶电泳对来自大毒蜥响尾蛇毒液的一种主要蛋白质毒素进行了检测。该毒素通过离子交换色谱法被分离成几种同工型，并自发解离成游离的酸性和碱性亚基，这与响尾蛇毒素的行为相似。用响尾蛇毒素免疫家兔产生的抗血清在酶联免疫吸附试验中与完整的大毒蜥响尾蛇毒素同工型及其碱性亚基发生强烈反应，并在双向免疫扩散凝胶中与完整的响尾蛇毒素形成同一性沉淀线。这些结果表明，大毒蜥响尾蛇毒素与南美响尾蛇毒液中的响尾蛇毒素具有高度同源性。
