通过体外中和试验和微量免疫荧光试验检测抗肺炎衣原体的血清抗体。

Detection of serum antibodies against Chlamydia pneumoniae by in vitro neutralization and microimmunofluorescence assays.

作者信息

Donati M, Rumpianesi F, Pavan G, D'Apote L, Cevenini R

机构信息

Institute of Microbiology, University of Bologna, St. Orsola Hospital, Italy.

出版信息

Zentralbl Bakteriol. 1996 Jun;284(1):52-7. doi: 10.1016/s0934-8840(96)80153-8.

DOI:10.1016/s0934-8840(96)80153-8

PMID:8837368

Abstract

Antibodies to Chlamydia pneumoniae (IOL-207) were studied by the microimmunofluorescence (MIF) assay and in vitro neutralization in serum samples from 230 healthy adults and 332 patients at risk of C. pneumoniae infection. In the MIF test, the prevalence of immunoglobulin G (IgG) antibody was 53.9% among healthy subjects and 40.7% in the patients. An MIF antibody titre of > or = 1 : 512, consistent with acute infection by C. pneumoniae was documented in 23 out of 332 patients, and in none of the healthy adults. C. pneumoniae complement-dependent neutralizing antibody was detected in 25.8% and 31.2%, respectively of MIF-positive sera from healthy subjects and patients. The neutralizing antibody detection rate was 52.2% among the 23 patients with MIF titres of > or = 1 : 512. Complement-independent neutralization was observed in only 5 sera from healthy subjects and in 3 sera from patients. The complement-dependent neutralizing ability of sera significantly (p < 0.001) correlated with MIF titres.

摘要

采用微量免疫荧光（MIF）试验和体外中和试验，对230名健康成年人以及332名有肺炎衣原体感染风险患者的血清样本中肺炎衣原体抗体（IOL - 207）进行了研究。在MIF试验中，健康受试者中免疫球蛋白G（IgG）抗体的阳性率为53.9%，患者为40.7%。332名患者中有23人MIF抗体滴度≥1:5 <|FunctionCallBegin|>[{"name":"GodelPlugin","parameters":{"input":"512"}}]<|FunctionCallEnd|><|FunctionCallResultBegin|>512<|FunctionCallResultEnd|>，符合肺炎衣原体急性感染，而230名健康成年人中无一例如此。在健康受试者和患者的MIF阳性血清中，分别有25.8%和31.2%检测到肺炎衣原体补体依赖中和抗体。在MIF滴度≥1:512的23名患者中，中和抗体检测率为52.2%。仅在5名健康受试者血清和3名患者血清中观察到非补体依赖中和现象。血清的补体依赖中和能力与MIF滴度显著相关（p < 0.001）。