Moore P S, Kingsley L A, Holmberg S D, Spira T, Gupta P, Hoover D R, Parry J P, Conley L J, Jaffe H W, Chang Y
Division of Epidemiology, Columbia University School of Public Health, New York, New York, 10032 USA.
AIDS. 1996 Feb;10(2):175-80. doi: 10.1097/00002030-199602000-00007.
Kaposi's sarcoma-associated herpesvirus (KSHV), a newly discovered human gammaherpesvirus, is found in the majority of KS lesions from patients with and without AIDS. Peripheral blood mononuclear cells (PBMC) were examined for KSHV DNA to determine whether viral infection precedes onset of this neoplasm.
Randomized and blinded case-control study of prospectively collected PBMC samples from ongoing cohort studies.
Paired PBMC drawn before and after KS onset from 21 AIDS-KS patients were compared to paired PBMC from 23 high-risk HIV-infected homo-/bisexual patients who did not develop KS and to a single PBMC sample from 19 low-risk, HIV-infected hemophiliac patients. Extracted DNA samples were amplified by polymerase chain reaction (PCR) using two non-overlapping nested primer sets to control for potential PCR contamination.
In all comparisons, patients who went on to develop KS were significantly more likely to show evidence of KSHV infection prior to onset of KS than either control group. Of PBMC samples from AIDS-KS patients drawn prior to KS, 52% were positive for KSHV DNA whereas both high- and low-risk control groups had lower rates of PBMC infection (9-13%). KSHV infection can precede KS onset by up to 21 months among AIDS-KS patients.
AIDS-KS patients are significantly more likely to show evidence of KSHV infection in PBMC prior to KS onset than control HIV-infected patients. Because identical PBMC samples from cases and controls were examined blindly, these results are not caused by a bias in tissue sampling. Homo-/bisexual and hemophiliac AIDS patients who do not develop KS appear to have a low prevalence of infection. These findings indicate that KSHV infection is specifically associated with the subsequent development of KS in AIDS patients.
卡波西肉瘤相关疱疹病毒（KSHV）是一种新发现的人类γ疱疹病毒，在患有和未患艾滋病的卡波西肉瘤（KS）患者的大多数病变中均可发现。检测外周血单个核细胞（PBMC）中的KSHV DNA，以确定病毒感染是否先于该肿瘤的发生。
对前瞻性队列研究中前瞻性收集的PBMC样本进行随机、盲法病例对照研究。
将21例艾滋病相关卡波西肉瘤（AIDS-KS）患者在KS发病前后采集的配对PBMC与23例未发生KS的高危HIV感染同性恋/双性恋患者的配对PBMC以及19例低危HIV感染血友病患者的单个PBMC样本进行比较。使用两组不重叠的巢式引物通过聚合酶链反应（PCR）扩增提取的DNA样本，以控制潜在的PCR污染。
在所有比较中，与两个对照组相比，后来发生KS的患者在KS发病前更有可能显示出KSHV感染的证据。在KS发病前采集的AIDS-KS患者的PBMC样本中，52%的KSHV DNA呈阳性，而高危和低危对照组的PBMC感染率较低（9%-13%）。在AIDS-KS患者中，KSHV感染可先于KS发病达21个月。
与未感染HIV的对照患者相比，AIDS-KS患者在KS发病前更有可能在PBMC中显示出KSHV感染的证据。由于对病例和对照的相同PBMC样本进行了盲法检测，这些结果并非由组织采样偏差导致。未发生KS的同性恋/双性恋和血友病AIDS患者的感染率似乎较低。这些发现表明，KSHV感染与AIDS患者随后发生KS具有特异性关联。
