Bernstein S L, Borst D E, Neuder M E, Wong P
National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Genomics. 1996 Mar 15;32(3):301-8. doi: 10.1006/geno.1996.0123.
The primate fovea is the region of the retina responsible for acute vision. This region constitutes less than 5% of the total area of the retina and has not been intensely studied at the molecular level. As a first step in the molecular characterization of the fovea, we have constructed a primary human fovea cDNA library. Experiments confirm that our cDNA library reflects a nonbiased distribution of foveal expressed sequences. Single-pass sequencing was performed on 209 randomly isolated clones from this library. Analysis of the sequences generated reveals that the distributions of fovea clones with either human mitochondrial gene sequences or repetitive elements are different than those observed in cDNA libraries made from other tissues. A significant number of the fovea expressed sequence tags (ESTs) (88, 42.1%) represent novel human ESTs. This suggests that the library will be useful in identifying new human genes. Northern analysis of specific fovea ESTs defined in this study suggests that there are significant quantitative differences in gene expression that distinguish the fovea from the rest of the retina.
灵长类动物的中央凹是视网膜中负责敏锐视觉的区域。该区域占视网膜总面积的比例不到5%，尚未在分子水平上进行深入研究。作为对中央凹进行分子特征描述的第一步，我们构建了一个人类中央凹初级cDNA文库。实验证实，我们的cDNA文库反映了中央凹表达序列的无偏差分布。对该文库中随机分离的209个克隆进行了单通道测序。对所产生序列的分析表明，含有人类线粒体基因序列或重复元件的中央凹克隆的分布与从其他组织构建的cDNA文库中观察到的分布不同。大量的中央凹表达序列标签（EST）（88个，占42.1%）代表了新的人类EST。这表明该文库将有助于鉴定新的人类基因。对本研究中定义的特定中央凹EST进行的Northern分析表明，基因表达存在显著的定量差异，这些差异将中央凹与视网膜的其他部分区分开来。
