• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Single pass sequencing of a unidirectional human fetal heart cDNA library to discover novel genes of the cardiovascular system.

作者信息

Hwang D M, Hwang W S, Liew C C

机构信息

Laboratory for Molecular Cardiology, University of Toronto, Ontario, Canada.

出版信息

J Mol Cell Cardiol. 1994 Oct;26(10):1329-33. doi: 10.1006/jmcc.1994.1151.

DOI:10.1006/jmcc.1994.1151
PMID:7869393
Abstract

A human fetal heart cDNA library was constructed in the lambda gt22A expression vector. Polymerase chain reaction (PCR) was used to amplify the cDNA inserts. PCR products were purified and used in cycle sequencing reactions in the presence of a fluorescein-conjugated primer and electrophoresed on a Pharmacia A.L.F. Sequencer. Partial cDNA sequences, or expressed sequence tags (ESTs) were searched against the Genbank and EMBL databases to identify novel genes expressed in the human cardiovascular system.

摘要

相似文献

1
Single pass sequencing of a unidirectional human fetal heart cDNA library to discover novel genes of the cardiovascular system.
J Mol Cell Cardiol. 1994 Oct;26(10):1329-33. doi: 10.1006/jmcc.1994.1151.
2
Human CD34+ cell EST database: single-pass sequencing of 402 clones from a directional cDNA library.人类CD34 +细胞EST数据库：来自定向cDNA文库的402个克隆的单通道测序
Exp Hematol. 1996 Apr;24(5):605-12.
3
Characterization of gene expression in human trabecular meshwork using single-pass sequencing of 1060 clones.使用1060个克隆的单通道测序对人小梁网中的基因表达进行表征。
Invest Ophthalmol Vis Sci. 2000 Nov;41(12):3678-93.
4
[Finding of new FCC1/HN antigenic expressed sequence tag(ESTs) of Plasmodium falciparum].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(6):363-6.
5
[Construction and preliminary screening of a forward-subtracted cDNA library for differentially expressed genes in rat liver of prothrombotic state].[构建并初步筛选前血栓形成状态大鼠肝脏中差异表达基因的正向消减cDNA文库]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Nov;36(6):761-4.
6
Characterization of a novel gene encoding zinc finger domains identified from expressed sequence tags (ESTs) of a human heart cDNA database.从人心脏cDNA数据库的表达序列标签（EST）中鉴定出的一种编码锌指结构域的新基因的特征分析。
J Mol Cell Cardiol. 1998 Nov;30(11):2365-75. doi: 10.1006/jmcc.1998.0797.
7
Characterization of a human fovea cDNA library and regional differential gene expression in the human retina.人中央凹cDNA文库的特征分析及人视网膜区域差异基因表达
Genomics. 1996 Mar 15;32(3):301-8. doi: 10.1006/geno.1996.0123.
8
Construction of a normalized directionally cloned cDNA library from adult heart and analysis of 3040 clones by partial sequencing.构建成年心脏标准化定向克隆cDNA文库并通过部分测序对3040个克隆进行分析。
Genomics. 1996 Jul 1;35(1):231-5. doi: 10.1006/geno.1996.0344.
9
[Preliminary screening of a reverse-subtracted cDNA library for differentially expressed genes in rat liver of prothrombotic state].[对大鼠血栓前状态肝脏中差异表达基因的反向消减cDNA文库进行初步筛选]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Jul;35(4):455-9.
10
Cataloging of the genes expressed in human keratinocytes: analysis of 607 randomly isolated cDNA sequences.人类角质形成细胞中表达基因的编目：对607个随机分离的cDNA序列的分析。
Biochem Biophys Res Commun. 1994 Jul 29;202(2):976-83. doi: 10.1006/bbrc.1994.2025.

引用本文的文献

1
Guardian of Genetic Messenger-RNA-Binding Proteins.遗传信使核糖核酸结合蛋白的守护者
Biomolecules. 2016 Jan 6;6(1):4. doi: 10.3390/biom6010004.
2
Association of CELF2 polymorphism and the prognosis of nasopharyngeal carcinoma in southern Chinese population.CELF2基因多态性与中国南方人群鼻咽癌预后的相关性
Oncotarget. 2015 Sep 29;6(29):27176-86. doi: 10.18632/oncotarget.4870.
3
The importance of CELF control: molecular and biological roles of the CUG-BP, Elav-like family of RNA-binding proteins.CELF 调控的重要性：CUG-BP、Elav 样 RNA 结合蛋白家族的分子和生物学作用。
Wiley Interdiscip Rev RNA. 2012 Jan-Feb;3(1):104-21. doi: 10.1002/wrna.107. Epub 2011 Aug 17.
4
The neurofibromatosis type I pre-mRNA is a novel target of CELF protein-mediated splicing regulation.神经纤维瘤病 I 型前体 mRNA 是 CELF 蛋白介导的剪接调控的新靶标。
Nucleic Acids Res. 2010 Jan;38(1):253-64. doi: 10.1093/nar/gkp766. Epub 2009 Oct 23.
5
Global gene expression profiling of end-stage dilated cardiomyopathy using a human cardiovascular-based cDNA microarray.利用基于人类心血管的cDNA微阵列对终末期扩张型心肌病进行全基因组表达谱分析。
Am J Pathol. 2002 Jun;160(6):2035-43. doi: 10.1016/S0002-9440(10)61153-4.
6
The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing.CELF 家族的 RNA 结合蛋白与细胞特异性及发育调控的可变剪接有关。
Mol Cell Biol. 2001 Feb;21(4):1285-96. doi: 10.1128/MCB.21.4.1285-1296.2001.
7
Identification, characterization, and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library.来自斑马鱼胚胎心脏cDNA文库的表达序列标签的鉴定、表征及定位
Genome Res. 2000 Dec;10(12):1915-27. doi: 10.1101/gr.10.12.1915.
8
Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha.第三个α类缺氧诱导因子亚基HIF3α的分子特征及染色体定位
Gene Expr. 1998;7(3):205-13.
9
Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest).人心脏cDNA文库的构建及心血管相关基因的鉴定（CVBest）。
Mol Cell Biochem. 1997 Jul;172(1-2):81-7.
10
Identification of a cDNA encoding 6-phosphogluconate dehydrogenase from a human heart cDNA library.从人心脏cDNA文库中鉴定编码6-磷酸葡萄糖酸脱氢酶的cDNA。
Biochem Genet. 1996 Oct;34(9-10):367-73. doi: 10.1007/BF00554412.