Babál P, Gardner W A
Department of Pathology, Comenius University, Bratislava, Slovakia.
Histol Histopathol. 1996 Jul;11(3):621-31.
A new sialic acid-specific lectin from the colonic parasite of squirrel monkeys Tritrichomonas mobilensis (TML) was tested on human and mouse tissues for histochemical staining properties. There were no substantial differences in reactivity between frozen and formalin fixed paraffin sections. TML staining was blocked by preincubation with sialic acid or by sialidase digestion. TML/anti-TML antibody histochemistry was identical with the TML-gold technique. The staining pattern was not blood group dependent. TML stained strongly the luminal membranes of normal vascular endothelium as well as endothelial neoplasms. Lymphatic vessels and capillaries of kidney glomeruli and lung alveolar septi were negative or only slightly positive. In parenchymatous organs luminal membrane positivity was dominant, preferably of cells lining ducts. Weak fine-granular cytoplasmic and basolateral membrane staining was also observed. Umbrella cells in transitional epithelium and basal layers of squamous epithelia showed strong reactivity with cell membranes. Mucin in respiratory epithelium was positive whereas gastrointestinal mucins failed to stain uniformly. Erythrocytes and most white blood cell types showed distinct membrane positivity. Acetylation or alkaline O-deacetylation of tissue sections did not substantially change TML reactivity. Oxidation, however, completely blocked TML staining except for respiratory epithelium and colonic mucin.
对从松鼠猴结肠寄生虫活动三毛滴虫（TML）中提取的一种新的唾液酸特异性凝集素进行了人及小鼠组织的组织化学染色特性测试。冷冻切片和福尔马林固定石蜡切片之间的反应性没有实质性差异。用唾液酸预孵育或用唾液酸酶消化可阻断TML染色。TML/抗TML抗体组织化学与TML-金技术相同。染色模式不依赖血型。TML强烈染色正常血管内皮以及内皮肿瘤的腔面膜。肾小球和肺泡隔的淋巴管和毛细血管呈阴性或仅轻微阳性。在实质器官中，腔面膜阳性占主导，主要是导管内衬细胞。还观察到弱的细颗粒状细胞质和基底外侧膜染色。移行上皮中的伞细胞和鳞状上皮的基底层与细胞膜有强烈反应。呼吸道上皮中的粘蛋白呈阳性，而胃肠道粘蛋白未能均匀染色。红细胞和大多数白细胞类型显示出明显的膜阳性。组织切片的乙酰化或碱性O-脱乙酰化并没有实质性改变TML的反应性。然而，氧化完全阻断了TML染色，呼吸道上皮和结肠粘蛋白除外。
