Komuro T, Tokui K, Zhou D S
Department of Basic Human Sciences, Waseda University School of Human Sciences, Saitama, Japan.
Histol Histopathol. 1996 Jul;11(3):769-86.
Observation of whole mount stretch preparations using the zinc-iodide-osmic acid method reveals a wide variety of interstitial cells in different tissue layers of the guinea-pig small intestine. And a subsequent electron-microscopic examination and survey of references makes clear that the interstitial cells of Cajal (ICC) depicted in original drawings of Cajal are heterogeneous and correspond to different types of interstitial cells. The myenteric ICC are characterized by long dichotomous branching processes which constitute cellular networks independent from the nerve plexus and form many gap junctions at their tips. Their ultrastructure is similar to that of fibroblasts and they have no basal lamina. The myenteric ICC show strong immunoreactivity for vimentin and the c-kit receptor, and probably correspond to the intestinal pacemaker cells. Within the circular muscle layer, ICC are represented by the cells that are closely associated with fine nerve bundles. The ICC have various shapes, ranging from bipolar to stellate, depending on the running pattern of the nerve fibers that they are associated with. They show fibroblast-like ultrastructure and have no basal lamina. They form gap junctions with smooth muscle cells and are immunoreactive for vimentin. On the other hand, ICC associated with the deep muscular plexus described in the guinea-pig by Cajal could not be clearly identified. However, it is suggested that the ICC in this location may correspond to glycogen-rich cells possessing a basal lamina. Although they show a fairly well-developed rough endoplasmic reticulum, Golgi apparatus and immunoreactivity for vimentin, ICC of the deep muscular plexus are probably specialized smooth muscle cells in nature.
使用碘化锌锇酸法对整装伸展标本进行观察，可发现豚鼠小肠不同组织层中有多种间质细胞。随后的电子显微镜检查和参考文献查阅表明， Cajal原始绘图中描绘的Cajal间质细胞（ICC）具有异质性，对应于不同类型的间质细胞。肌间ICC的特征是具有长的二叉分支突起，这些突起构成独立于神经丛的细胞网络，并在其末端形成许多缝隙连接。它们的超微结构与成纤维细胞相似，且没有基膜。肌间ICC对波形蛋白和c-kit受体表现出强烈的免疫反应性，可能对应于肠道起搏细胞。在环形肌层内，ICC由与细神经束紧密相关的细胞代表。ICC具有各种形状，从双极到星状不等，这取决于它们所关联的神经纤维的走行模式。它们表现出成纤维细胞样的超微结构，没有基膜。它们与平滑肌细胞形成缝隙连接，对波形蛋白有免疫反应性。另一方面，Cajal在豚鼠中描述的与深肌丛相关的ICC无法清晰识别。然而，有人认为这个位置的ICC可能对应于具有基膜的富含糖原的细胞。尽管它们显示出相当发达的粗面内质网、高尔基体以及对波形蛋白的免疫反应性，但深肌丛的ICC本质上可能是特化的平滑肌细胞。
